Highly Selective Cerebral ATP Assay Based on Micrometer Scale Ion Current Rectification at Polyimidazolium-Modified Micropipettes

被引：61

作者：

Zhang, Kailin ^{[1
,2
]}

He, Xiulan ^{[2
,3
]}

Liu, Yang ^{[2
]}

Yu, Ping ^{[2
,3
]}

Fei, Junjie ^{[1
]}

Mao, Lanqun ^{[2
,3
]}

机构：

[1] Xiangtan Univ, Coll Chem, Minist Educ, Key Lab Environm Friendly Chem & Applicat, Xiangtan 411105, Hunan, Peoples R China

[2] Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 12期

关键词：

RAT STRIATUM; RECOGNITION; TIME; BIOSENSOR; GLUCOSE; ENERGY; FLOW;

D O I：

10.1021/acs.analchem.7b01218

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Development of new principles and methods for cerebral ATP assay is highly imperative not only for determining ATP dynamics in brain but also for understanding physiological, and pathological processes related to ATP. Herein, we for the first time demonstrate that micrometer scale ion current rectification (MICR) at a polyimidazolium brush-modified micropipette can be used as the signal transduction output for the cerebral ATP assay with a high selectivity. The rationale for ATP assay is essentially based on the competitive binding ability between positively charged polyimidazolium and ATP toward negatively charged ATP aptamer. The method is well responsive to ATP with a good linearity within a concentration range from 5 nM to 100 nM, and high selectivity toward ATP. These properties essentially enable the method to determine the cerebral ATP by combining in vivo microdialysis. The basal dialysate level of ATP in rat brain cortex is determined to be 11.32 +/- 2.36 nM (n = 3). This study demonstrates that the MICR-based sensors could be potentially used for monitoring neurochemicals in cerebral systems.

引用

页码：6794 / 6799

页数：6