Molecular characterization of FOX-4, a new ampC-type plasmid-mediated β-lactamase from an Escherichia coli strain isolated in Spain

被引:45
作者
Bou, G
Oliver, A
Ojeda, M
Monzón, C
Martínez-Beltrán, J
机构
[1] Hosp Ramon y Cajal, Microbiol Serv, E-28034 Madrid, Spain
[2] Hosp Insular, Microbiol Serv, Las Palmas Gran Canaria, Spain
关键词
D O I
10.1128/AAC.44.9.2549-2553.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A clinical strain of Escherichia coli (Ec GCE) displayed resistance to cefoxitin, cefotetan, cefotaxime, and ceftazidime. Susceptibility was not restored by the addition of clavulanic acid. Two beta-lactamases with apparent pIs of 5.4 and 6.4 were identified; the beta-lactamase with a pI of 6.4 was transferred by conjugation and associated with a 40-kb plasmid, Analysis of the nucleotide sequence showed a new ampC beta-lactamase gene that is closely related to those encoding the FOX-3, FOX-2, and FOX-1 beta-lactamases but whose product has four novel amino acid mutations, at positions 11 (M-->T), 43 (A-->E), 233 (V-->A), and 280 (Y-->H). This first cephamycinase from Spain was named FOX-4.
引用
收藏
页码:2549 / 2553
页数:5
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