L-arginine-nitric oxide kinetics in nonnal and type 2 diabetic subjects -: A stable-labelled 15N arginine approach

被引:46
作者
Avogaro, A
Toffolo, G
Kiwanuka, E
de Kreutzenberg, SV
Tessari, P
Cobelli, C
机构
[1] Univ Padua, Dept Informat Engn, Padua, Italy
[2] Univ Padua, Dept Metab Dis, Padua, Italy
关键词
D O I
10.2337/diabetes.52.3.795
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Defective endothelium is a key event in the development of atherosclerosis in diabetes: alteration of the L-arginine-nitric oxide (NO) pathway has been suggested. We propose a modeling approach of the L-arginine-NO pathway in vivo in both control and type 2 diabetic subjects based on the intravenous bolus injection Of L-[N-15]arginine and subsequent noncompartmental and compartmental model analysis Of L-[N-15] arginine in plasma and [N-15]nitrate in the urine. No differences in arginine kinetics were observed between normal subjects and diabetic patients. [N-15]nitrates were detectable up to 48 It from the L-(15)[N]arginine administration; no differences were found in the tracer-to-tracee ratio in each urine collection. However, the NO synthesis in plasma from arginine was lower (P = 0.05 for the noncompartmental and 0.1208 for the compartmental analysis, by Mann-Whitney test) in diabetic patients than in control subjects when expressed both in absolute terms (50% decrease) and as percentage of NO turnover (30% decrease). This new modeling approach Of L-arginine-NO pathway provides a detailed picture of arginine kinetics and nitrate metabolism. From our data, it appears that noncomplicated type 2 diabetic patients have a decreased conversion of arginine to NO.
引用
收藏
页码:795 / 802
页数:8
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