Helicobacter pylori supernatants cause epithelial cytoskeletal disruption that is bacterial strain and epithelial cell line dependent but not toxin VacA dependent

被引：19

作者：

Bebb, JR

Letley, DP

Rhead, JL

Atherton, JC ^{[1
]}

机构：

[1] Univ Nottingham, Univ Nottingham Hosp, Div Gastroenterol, Nottingham NG7 2UH, England

[2] Univ Nottingham, Univ Nottingham Hosp, Inst Infect Immun & Inflammat, Nottingham NG7 2UH, England

来源：

INFECTION AND IMMUNITY | 2003年 / 71卷 / 06期

关键词：

D O I：

10.1128/IAI.71.6.3623-3627.2003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We show here that Helicobacter pylori broth culture supernatants disrupt the actin cytoskeleton of epithelial cell lines, leading to cell rounding and apoptosis through anoikis. We demonstrate that there are marked quantitative differences between strains and that there are different cell line sensitivities. By constructing VacA null isogenic mutants, we show that the effect is not due to the vacuolating cytotoxin.

引用

收藏

页码：3623 / 3627

页数：5

相关论文

共 31 条

[1] MOSAICISM IN VACUOLATING CYTOTOXIN ALLELES OF HELICOBACTER-PYLORI - ASSOCIATION OF SPECIFIC VACA TYPES WITH CYTOTOXIN PRODUCTION AND PEPTIC-ULCERATION [J].

ATHERTON, JC ;

CAO, P ;

PEEK, RM ;

TUMMURU, MKR ;

BLASER, MJ ;

COVER, TL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (30) :17771-17777

[2]

Atherton JC, 1998, BRIT MED BULL, V54, P105

[3] Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori [J].

Atherton, JC ;

Peek, RM ;

Tham, KT ;

Cover, TL ;

Blaser, MJ .

GASTROENTEROLOGY, 1997, 112 (01) :92-99

[4]

Atherton John C., 1997, P133

[5] cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors [J].

Censini, S ;

Lange, C ;

Xiang, ZY ;

Crabtree, JE ;

Ghiara, P ;

Borodovsky, M ;

Rappuoli, R ;

Covacci, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14648-14653

[6] Apoptosis in gastric epithelial cells is induced by Helicobacter pylori and accompanied by increased expression of BAK [J].

Chen, G ;

Sordillo, EM ;

Ramey, WG ;

Reidy, J ;

Holt, PR ;

Krajewski, S ;

Reed, JC ;

Blaser, MJ ;

Moss, SF .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 239 (02) :626-632

[7]

CORREA P, 1995, AM J SURG PATHOL, V19, P37

[8]

COVER TL, 1994, J BIOL CHEM, V269, P10566

[9] Bacteroides fragilis enterotoxin induces cytoskeletal changes and surface blebbing in HT-29 cells [J].

Donelli, G ;

Fabbri, A ;

Fiorentini, C .

INFECTION AND IMMUNITY, 1996, 64 (01) :113-119

[10] CHARACTERIZATION AND PRESUMPTIVE IDENTIFICATION OF HELICOBACTER-PYLORI ISOLATES FROM RHESUS-MONKEYS [J].

DRAZEK, ES ;

DUBOIS, A ;

HOLMES, RK .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (07) :1799-1804

← 1 2 3 4 →