Glucose-dependent insulin release from genetically engineered K cells

被引：216

作者：

Cheung, AT

Dayanandan, B

Lewis, JT

Korbutt, GS

Rajotte, RV

Bryer-Ash, M

Boylan, MO

Wolfe, MM

Kieffer, TJ ^{[1
]}

机构：

[1] Univ Alberta, Dept Med, Edmonton, AB T6G 2S2, Canada

[2] Univ Alberta, Dept Physiol, Edmonton, AB T6G 2S2, Canada

[3] Univ Alberta, Dept Surg, Edmonton, AB T6G 2S2, Canada

[4] enGene Inc, Edmonton, AB T6G 2T5, Canada

[5] Univ Tennessee, Dept Med, Memphis, TN 38103 USA

[6] Boston Med Ctr, Gastroenterol Sect, Boston, MA 02118 USA

来源：

SCIENCE | 2000年 / 290卷 / 5498期

关键词：

D O I：

10.1126/science.290.5498.1959

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genetic engineering of non-beta cells to release insulin upon feeding could be a therapeutic modality for patients with diabetes. A tumor-derived K-cell line was induced to produce human insulin by providing the cells with the human insulin gene linked tb the 5'-regulatory region of the gene encoding glucose-dependent insulinotropic polypeptide (GIP). Mice expressing this transgene produced human insulin specifically in gut K cells. This insulin protected the mice from developing diabetes and maintained glucose tolerance after destruction of the native insulin-producing beta cells.

引用

页码：1959 / 1962

页数：4

共 24 条

[1] Toward the biologic release of human insulin from skeletal muscle [J].