Characterization of the yeast ecosystem in grape must and wine using real-time PCR

被引：118
作者：
Zott, K. [1 ,2 ]
Claisse, O. [2 ]
Lucas, P. [2 ]
Coulon, J.
Lonvaud-Funel, A. [2 ]
Masneuf-Pomarede, I. [1 ,2 ]
机构：
[1] ENITA Bordeaux, F-33175 Gradignan, France
[2] Univ Bordeaux, ISVV, INRA UMR 1219, F-33882 Villenave Dornon, France
来源：
FOOD MICROBIOLOGY | 2010年 / 27卷 / 05期
关键词：
Real-time PCR; Non-Saccharomyces yeasts ecology; Grape must; Wine; NON-SACCHAROMYCES YEASTS; INTERNAL TRANSCRIBED SPACERS; RIBOSOMAL-RNA GENE; QUANTITATIVE PCR; CANDIDA-ZEMPLININA; RAPID DETECTION; RFLP ANALYSIS; FERMENTATION; IDENTIFICATION; CEREVISIAE;
D O I：
10.1016/j.fm.2010.01.006
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The complex microbial ecosystem of grape must and wine harbours a wide diversity of yeast species. Specific oligonucleotide primers for real-time quantitative PCR(QPCR) were designed to analyse several important non-Saccharomyces yeasts (Issatchenkia orientalis, Metschnikowia pulcherrima, Torulaspora delbrueckii, Candida zemplinina and Hanseniaspora spp.) and Saccharomyces spp. in fresh wine must, during fermentation and in the finished wine. The specificity of all primer couples for their target yeast species were validated and the QPCR methods developed were compared with a classic approach of colony identification by RFLP-ITS-PCR on cultured samples. Once the methods had been developed and validated, they were used to study these non-Saccharomyces yeasts in wine samples and to monitor their dynamics throughout the fermentation process. This study confirms the usefulness and the relevance of QPCR for studying non-Saccharomyces yeasts in the complex yeast ecosystem of grape must and wine. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：559 / 567
页数：9
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