Preparation of thermosensitiye polymer magnetic particles and their application in protein separations

被引：8
作者：
Luo, Ling [1 ]
Zhang, Hong-Su [1 ]
Liu, Yan [1 ]
Ha, Wei [2 ]
Li, Luo-Hao [1 ]
Gong, Xiao-Lei [1 ]
Li, Bang-Jing [2 ]
Zhang, Sheng [1 ]
机构：
[1] Sichuan Univ, Polymer Res Inst, State Key Lab Polymer Mat Engn, Chengdu 610065, Peoples R China
[2] Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China
来源：
JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2014年 / 435卷
基金：
中国国家自然科学基金;
关键词：
Self-assembly; Magnetic particles; Host-guest recognition; Lysozyme; BOVINE SERUM-ALBUMIN; SELF-ASSEMBLED MONOLAYERS; CONFORMATIONAL-CHANGES; NANOMAGNETIC PARTICLES; LYSOZYME ADSORPTION; ALPHA-CYCLODEXTRIN; INCLUSION COMPLEX; OXIDE SURFACES; NANOPARTICLES; DESORPTION;
D O I：
10.1016/j.jcis.2014.07.007
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
This paper presents a kind of thermoresponsive polymeric magnetic particles for protein separations. The magnetofluids were directly encapsulated in hollow particles constructed by self-assembly of rod-coil poly(ethylene glycol)-poly(N-isopropylacrylamide)/alpha-cyclodextrin (PEG-PNIPAM/alpha-CD) complexes. The resulting particles showed reversible protein absorption/desorption capacity because the reversible thermo-sensitivity of PNIPAM. Above the lower critical solution temperature (LCST) of PNIPAM, these particles showed high absorptive capacities and adsorption was done at lower temperature. The protein-laden particles are readily removed from the feed solution in a magnetic field. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：99 / 104
页数：6
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