Exploiting the Anti-Aggregation of Gold Nanostars for Rapid Detection of Hand, Foot, and Mouth Disease Causing Enterovirus 71 Using Surface-Enhanced Raman Spectroscopy

被引:40
作者
Reyes, Miguel [1 ]
Piotrowski, Marek [2 ,3 ]
Ang, Swee Kim [4 ]
Chan, Jingqi [5 ]
He, Shuai [6 ]
Chu, Justin Jang Hann
Kah, James Chen Yong [6 ,7 ]
机构
[1] Natl Univ Singapore, Dept Mat Sci & Engn, 9 Engn Dr 1,Blk EA,03-09, Singapore 117575, Singapore
[2] Polish Acad Sci, Jerzy Haber Inst Catalysis & Surface Chem, Niezapominajek 8, PL-30239 Krakow, Poland
[3] Int Iberian Nanotechnol Lab, Ave Mestre Jose Veiga, P-4715330 Braga, Portugal
[4] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol & Immunol, 5 Sci Dr 2,Blk MD4,Level 5, Singapore 117597, Singapore
[5] Temasek Jr Coll, 22 Bedok South Rd, Singapore 469278, Singapore
[6] Natl Univ Singapore, Dept Biomed Engn, 4 Engn Dr 3,Blk E4,04-08, Singapore 117583, Singapore
[7] Natl Univ Singapore, NUS Grad Sch Integrat Sci & Engn, Ctr Life Sci CeLS, 05-01,28 Med Dr, Singapore 117456, Singapore
关键词
NANOPARTICLE AGGREGATION; SCATTERING SERS; PROTEIN CORONA; SILVER; IMMUNOASSAY; EV71; EPIDEMIOLOGY; STABILITY; MOLECULES; SUBSTRATE;
D O I
10.1021/acs.analchem.7b00066
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Enterovirus 71 (EV71) is a major public health threat that requires rapid point-of-care detection. Here, we developed a surface-enhanced Raman spectroscopy (SERS)-based scheme that utilized protein-induced aggregation of colloidal gold nanostars (AuNS) to rapidly detect EV71 without the need for fabricating a solid substrate, Raman labels or complicated sample handling. We used AuNS (hydrodynamic diameter, D-H of 105.12 +/- 1.13 nm) conjugated to recombinant scavenger receptor class B, member 2 (SCARB2) protein with known affinity to EV71. In the absence of EV71, AuNS-SCARB2 aggregated in biological media and produced four enhanced Raman peaks at 390, 510, 670, and 910 cm(-1). In the presence of EV71, the three peaks at 510, 670, and 910 cm(-1) disappeared, while the peak at 390 cm(-1) diminished in intensity as the virus bound to AuNS-SCARB2 and prevented them from aggregation. These three peaks (510, 670, and 910 cm(-1)) were potential markers for specific detection of EV71 as their disappearance was not observable with a different dengue virus (DENY) as our control. Furthermore, the Raman measurements from colloidal SERS were more sensitive in probing the aggregation of AuNS-SCARB2 for detecting the presence of EV71 in protein-rich samples compared to UV-vis spectrum measurements. With this facile "anti-aggregation" approach, we were able to detect EV71 in protein-rich biological medium within 15 min with reasonable sensitivity of 10(7) pfu/mL and minimal sample preparation, making this translatable for point-of-care applications.
引用
收藏
页码:5373 / 5381
页数:9
相关论文
共 57 条
[31]   EV71 vaccine, a new tool to control outbreaks of hand, foot and mouth disease (HFMD) [J].
Mao, Qun-ying ;
Wang, Yiping ;
Bian, Lianlian ;
Xu, Miao ;
Liang, Zhenglun .
EXPERT REVIEW OF VACCINES, 2016, 15 (05) :599-606
[32]   Immobilised gold nanostars in a paper-based test system for surface-enhanced Raman spectroscopy [J].
Mehn, Dora ;
Morasso, Carlo ;
Vanna, Renzo ;
Bedoni, Marzia ;
Prosperi, Davide ;
Gramatica, Furio .
VIBRATIONAL SPECTROSCOPY, 2013, 68 :45-50
[33]   Nanoparticle colloidal stability in cell culture media and impact on cellular interactions [J].
Moore, Thomas L. ;
Rodriguez-Lorenzo, Laura ;
Hirsch, Vera ;
Balog, Sandor ;
Urban, Dominic ;
Jud, Corinne ;
Rothen-Rutishauser, Barbara ;
Lattuada, Marco ;
Petri-Fink, Alke .
CHEMICAL SOCIETY REVIEWS, 2015, 44 (17) :6287-6305
[34]   Surface-enhanced Raman scattering (SERS) detection of multiple viral antigens using magnetic capture of SERS-active nanoparticles [J].
Neng, Jing ;
Harpster, Mark H. ;
Wilson, William C. ;
Johnson, Patrick A. .
BIOSENSORS & BIOELECTRONICS, 2013, 41 :316-321
[35]   Probing single molecules and single nanoparticles by surface-enhanced Raman scattering [J].
Nie, SM ;
Emery, SR .
SCIENCE, 1997, 275 (5303) :1102-1106
[36]   Size correlation of optical and spectroscopic properties for gold nanoparticles [J].
Njoki, Peter N. ;
Lim, I-Im S. ;
Mott, Derrick ;
Park, Hye-Young ;
Khan, Bilal ;
Mishra, Suprav ;
Sujakumar, Ravishanker ;
Luo, Jin ;
Zhong, Chuan-Jian .
JOURNAL OF PHYSICAL CHEMISTRY C, 2007, 111 (40) :14664-14669
[37]   Crystal Structure of Human Enterovirus 71 [J].
Plevka, Pavel ;
Perera, Rushika ;
Cardosa, Jane ;
Kuhn, Richard J. ;
Rossmann, Michael G. .
SCIENCE, 2012, 336 (6086) :1274-1274
[38]   Evaluation of Nanoparticle Aggregation in Human Blood Serum [J].
Rausch, Kristin ;
Reuter, Anika ;
Fischer, Karl ;
Schmidt, Manfred .
BIOMACROMOLECULES, 2010, 11 (11) :2836-2839
[39]   Raman spectroscopy of proteins: a review [J].
Rygula, A. ;
Majzner, K. ;
Marzec, K. M. ;
Kaczor, A. ;
Pilarczyk, M. ;
Baranska, M. .
JOURNAL OF RAMAN SPECTROSCOPY, 2013, 44 (08) :1061-1076
[40]   Rapid and sensitive detection of respiratory virus molecular signatures using a silver nanorod array SERS substrate [J].
Shanmukh, Saratchandra ;
Jones, Les ;
Driskell, Jeremy ;
Zhao, Yiping ;
Dluhy, Richard ;
Tripp, Ralph A. .
NANO LETTERS, 2006, 6 (11) :2630-2636