Heparin recovers AT1 receptor and its intracellular signal transduction in cultured vascular smooth muscle cells

被引：13

作者：

Hashimoto, T ^{[1
]}

Kihara, M

Sato, K

Imai, N

Tanaka, Y

Sakai, M

Tamura, K

Hirawa, N

Toya, Y

Kitamura, H

Umemura, S

机构：

[1] Yokohama City Univ, Grad Sch Med & Sch Med, Div Cellular Pathobiol, Dept Pathol, Yokohama, Kanagawa 232, Japan

[2] Yokohama City Univ, Grad Sch Med & Sch Med, Dept Med Sci & Cardiorenal Med, Yokohama, Kanagawa 232, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 01期

基金：

日本学术振兴会;

关键词：

vascular smooth muscle cells differentiation; heparin; signal transduction; angiotensin type 1 expression; mitogen-activated protein kinase activation;

D O I：

10.1016/j.febslet.2004.11.093

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although vascular smooth muscle cells (VSMCs) are widely used in cardiovascular research, their phenotypic change under various culture conditions is problematic to evaluate the experimental results obtained. The levels of angiotensin (Ana) type 1/2 (ATI/AT2) receptors as well as contractile and structural proteins are degraded through culture passages. The present study demonstrated that heparin recovered Ang receptors and differentiation markers, such as desmin, SM-22 and smooth muscle a-actin in VSMCs at the ninth passage. Heparin also potenciated Ang II-induced activation for ERK1/2 and p38. These results suggest a potential value of heparin-treated VSMCs as the model for analysis of Ang-inediated signal transduction under physiological condition. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：281 / 284

页数：4

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