Development a new method for the determination of paromomycin in trace amounts by fast Fourier continuous cyclic voltammetry at an Au microelectrode in a flowing system

For the first time the technique of fast continuous cyclic voltammetry is used as a highly sensitive detection method for the monitoring of paromomycin in a flow-injection system. A special computer-based numerical calculation method (using fast Fourier transformation) is introduced here for the enhancement of the analyte signal and noise reduction. This research includes the observation of the effects of various parameters (rest potential, sweep rate and delay time) on the sensitivity of the detection system. Furthermore, a potential waveform, consisting of the potential steps for cleaning, accumulation and the step for the potential ramp of the analyte, was applied to an An disk microelectrode in a continuous way. Eventually, the method was found to be linear for the concentration range of 160-160,000 pg/mL (r = 0.999) with a limit of detection (LOD) and quantitation (LOQ) 49.25 and 160 pg/mL, respectively. The stripping time was less than 300 ms, while the detection limit was about 10,000 times lower than that of the most sensitive reported method. As a result, the system illustrates the requisite accuracy, sensitivity, precision and selectivity to assay paromomycin in capsules. (C) 2006 Elsevier B.V. All rights reserved.