A new recombinant SsnA protein combined with aluminum hydroxide protects mouse against Streptococcus suis

被引：13

作者：

Gomez-Gascon, Lidia ^{[1
]}

Cardoso-Toset, Fernando ^{[1
]}

Shyrley Amarilla, Paola ^{[2
]}

Tarradas, Carmen ^{[1
]}

Carrasco, Librado ^{[2
]}

Olaya-Abril, Alfonso ^{[3
]}

Jimenez-Munguia, Irene ^{[3
]}

Rodriguez-Ortega, Manuel J. ^{[3
]}

Luque, Inmaculada ^{[1
]}

机构：

[1] Univ Cordoba, Dept Sanidad Anim, Cordoba 1407, Spain

[2] Univ Cordoba, Dept Anat & Anat Patol Comparada, Cordoba 1407, Spain

[3] Univ Cordoba, Dept Bioquim & Biol Mol, Cordoba 1407, Spain

来源：

VACCINE | 2014年 / 32卷 / 51期

关键词：

Streptococcus suis serotype 2; Adjuvants; Recombinant SsnA-ALOH vaccine; Mouse model; VACCINE ADJUVANTS; SUBUNIT VACCINE; IMMUNE-RESPONSE; SURFACE PROTEIN; SEROTYPE-2; MICE; INFECTION; PIGS; STRAINS; SWINE;

D O I：

10.1016/j.vaccine.2014.10.019

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An experimental challenge in a mouse model was used to select the most effective adjuvant in a vaccine formulation with the surface-anchored DNA-nuclease (SsnA). We used a protocol based on clinical, histopathological, bacterial kinetics and immune response against S. suis serotype 2 in infected animals. The three adjuvants used, aluminum hydroxide (ALOH), incomplete Freund's adjuvant (FIA), oil-in-water adjuvant (OW) showed a protective effect against death by S. suis serotype 2 in this mouse model, although aluminum hydroxide revealed as the best option. Subsequently, in a second experimental assay, we showed that a recombinant SsnA protein combined with ALOH as adjuvant allowed a significant decrease of clinical and lesional findings in animals, faster reduction of the bacteria from organs and a highest humoral response against S. suis after 3 days post-infection. The results show that this combination (rSsnA+AlOH) could be a good vaccine formulation against S. suis, although further studies are necessary to evaluate their use for swine and human species. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：6992 / 6999

页数：8

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