Vacuum deposited polyaniline thin films have been found to exhibit high sensitivity to microbiological species. The fabrication of polyaniline thin film sensing devices for detection of pathogenic (disease causing) microbiological species is reported. These sensors operate at roam temperature, are cost effective, have quick response and high sensitivity towards microbial species normally encountered in medical diagnostics. These sensors can be used for on line monitoring of the pathological area. We have carried out feasibility investigations on these sensors. The behavioral acceptance tests like sensitivity; specificity, response, decays and recovery time have been carried out on these devices. A possible mechanism for the working of these microbiological sensors is proposed.