Alpha cells, the main source of IL-1β in human pancreas

被引：28

作者：

Anquetil, Florence ^{[1
]}

Sabouri, Somayeh ^{[1
]}

Thivolet, Charles ^{[1
]}

Rodriguez-Calvo, Teresa ^{[1
]}

Zapardiel-Gonzalo, Jose ^{[1
]}

Amirian, Natalie ^{[1
]}

Schneider, Darius ^{[1
]}

Castillo, Ericka ^{[1
]}

Lajevardi, Yasaman ^{[1
]}

von Herrath, Matthias G. ^{[1
,2
]}

机构：

[1] La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA

[2] Novo Nordisk Diabet Res & Dev Ctr, Seattle, WA USA

来源：

JOURNAL OF AUTOIMMUNITY | 2017年 / 81卷

基金：

美国国家卫生研究院;

关键词：

Alpha cells; Interleukin-1; beta; Type; 1; diabetes; Pancreas; DIABETES-MELLITUS; RECEPTOR ANTAGONIST; HUMAN ISLETS; BETA-CELLS; INTERLEUKIN-1; GLUCOSE; RAT; EXPRESSION; THERAPY; RELEASE;

D O I：

10.1016/j.jaut.2017.03.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Interleukin-1 beta (IL-1 beta) is known to trigger beta cell dysfunction in vitro and could potentially play a role during the pathogenesis of type 1 diabetes and type 2 diabetes. However, several clinical trials attempting to block IL-1 beta function have had minimal success. We therefore re-investigated local expression of IL-1 beta in human diabetic and non-diabetic pancreata. We obtained pancreatic tissue sections from the Network for Pancreatic Organ Donors with Diabetes (nPOD) including non-diabetic (n = 9), non-diabetic auto-antibody positive (AAb+, n = 5), type I diabetes (n = 6), and type 2 diabetes (n = 6) donors. Islets were systematically investigated for the presence of IL-1 beta mRNA by in situ hybridization and IL-1 beta protein by indirect immunofluorescence. We found that intra-islet IL-1 beta was produced at comparable level in both non-diabetic and diabetic donors. Interestingly, the main source for IL-1 beta was alpha cells but not beta cells. Our findings call into question the role of IL-1 beta in the diabetic pancreas as it has been proposed in previous literature. Additionally, our results regarding the localization of IL-1 beta should lead to further investigation into the role of IL-1 beta in the physiology of pancreatic alpha cells. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：68 / 73

页数：6

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