Laccase-mediator system produced by Trametes hirsuta Bm-2 on lignocellulosic substrate improves dye decolorization

被引：14

作者：

Ancona-Escalante, Wendy ^{[1
]}

Tapia-Tussell, Raul ^{[2
]}

Pool-Yam, Luis ^{[1
]}

Can-Cauich, Abraham ^{[1
]}

Lizama-Uc, Gabriel ^{[1
]}

Solis-Pereira, Sara ^{[1
]}

机构：

[1] Inst Tecnol Merida, Dept Ingn Quim & Bioquim, Merida 97118, Yucatan, Mexico

[2] Ctr Invest Cient Yucatan AC, Unidad Energia Renovable, Carretera Sierra Papacal Chuburna Puerto,Km 5, Merida 97302, Yucatan, Mexico

来源：

3 BIOTECH | 2018年 / 8卷 / 07期

关键词：

Trametes hirsuta; Laccase; Indigo carmine; Redox mediators; Lignin; FUNGAL LACCASES; RECALCITRANT DYES; REDOX MEDIATORS; OXIDATION; LIGNIN; DEGRADATION; REMOVAL;

D O I：

10.1007/s13205-018-1323-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lignin is a source for obtaining natural phenols with high commercial value that can act as redox mediators enhancing effects in dye decolorization. In this study Trametes hirsuta Bm-2 was grown on wheat bran to produce laccases and phenol extracts (PE). Ultrafiltered phenols obtained at different times were evaluated in their potential as redox mediators of laccase activity and indigo carmin decolorization. Laccase activity (L) on ABTS increased up to 12.4 times with L/PE72 compared with laccase alone and L/PE48 showed the highest level of dye decolorization (97%) compared with laccase (12%). The chromatographic analysis by HPLC showed variation in the profile and concentration of phenols at different times of culture. Stability of the laccase mediator system (LMs) in dye decolorization was maintained over 3 months. Our results suggest the use of natural mediators as a strategy for improving efficiency in dye biodegradation by laccase-producing fungi.

引用

页数：8

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