Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex

被引：154
作者：
Jónsson, ZO
Jha, S
Wohlschlegel, JA
Dutta, A
机构：
[1] Univ Virginia, Sch Med, Charlottesville, VA 22908 USA
[2] Harvard Univ, Sch Med, Brigham & Womens Hosp, Boston, MA 02115 USA
来源：
MOLECULAR CELL | 2004年 / 16卷 / 03期
关键词：
D O I：
10.1016/j.molcel.2004.09.033
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Rvb1p and Rvb2p (or TIP48 and TIP49) nuclear ATP binding proteins are universally conserved in eukaryotes and essential for viability of yeasts. Rvbp associate with each other as a double hexamer, with YHR034c and with two complexes involved in chromatin remodeling, Ino80.com and Swr1.com. Loss of Rvb1p or Ino80p affects many yeast promoters similarly. Rvbp are not essential for the recruitment of Ino80p to promoters but are essential for the catalytic activity of Ino80.com. Loss of Rvbp leads to loss of the functionally critical Arp5p in Ino80.com. Rvb2p associates with Arp5p in vitro in a reaction dependent on the presence of ATP and Ino80p. Therefore, Rvbp are required for the structural and functional integrity of the Ino80 chromatin remodeling complex.
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收藏
页码：465 / 477
页数：13
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