A rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009

被引:20
作者
Chen, Yixin [1 ]
Xu, Feihai [1 ]
Gui, Xun [1 ]
Yang, Kunyu [1 ]
Wu, Xingrong [1 ]
Zheng, Qingbing [1 ]
Ge, Shengxiang [1 ]
Yuan, Quan [1 ]
Yeo, Anthony E. T. [1 ]
Zhang, Jun [1 ]
Guan, Yi [2 ]
Chen, Honglin [2 ]
Xia, Ningshao [1 ]
机构
[1] Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Sch Life Sci, Xiamen, Peoples R China
[2] Univ Hong Kong, State Key Lab Emerging Infect Dis, Dept Microbiol, Pokfulam, Hong Kong, Peoples R China
基金
中国国家自然科学基金;
关键词
Influenza A; Nucleoprotein; Rapid test; Dot-ELISA; H1N1; VIRUS; MONOCLONAL-ANTIBODIES; NUCLEOPROTEIN; SENSITIVITY; HUMANS;
D O I
10.1016/j.jviromet.2010.02.001
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:100 / 102
页数:3
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