A rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009

被引：20

作者：

Chen, Yixin ^{[1
]}

Xu, Feihai ^{[1
]}

Gui, Xun ^{[1
]}

Yang, Kunyu ^{[1
]}

Wu, Xingrong ^{[1
]}

Zheng, Qingbing ^{[1
]}

Ge, Shengxiang ^{[1
]}

Yuan, Quan ^{[1
]}

Yeo, Anthony E. T. ^{[1
]}

Zhang, Jun ^{[1
]}

Guan, Yi ^{[2
]}

Chen, Honglin ^{[2
]}

Xia, Ningshao ^{[1
]}

机构：

[1] Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Sch Life Sci, Xiamen, Peoples R China

[2] Univ Hong Kong, State Key Lab Emerging Infect Dis, Dept Microbiol, Pokfulam, Hong Kong, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 167卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Influenza A; Nucleoprotein; Rapid test; Dot-ELISA; H1N1; VIRUS; MONOCLONAL-ANTIBODIES; NUCLEOPROTEIN; SENSITIVITY; HUMANS;

D O I：

10.1016/j.jviromet.2010.02.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：100 / 102

页数：3

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