A rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009

被引:20
|
作者
Chen, Yixin [1 ]
Xu, Feihai [1 ]
Gui, Xun [1 ]
Yang, Kunyu [1 ]
Wu, Xingrong [1 ]
Zheng, Qingbing [1 ]
Ge, Shengxiang [1 ]
Yuan, Quan [1 ]
Yeo, Anthony E. T. [1 ]
Zhang, Jun [1 ]
Guan, Yi [2 ]
Chen, Honglin [2 ]
Xia, Ningshao [1 ]
机构
[1] Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Sch Life Sci, Xiamen, Peoples R China
[2] Univ Hong Kong, State Key Lab Emerging Infect Dis, Dept Microbiol, Pokfulam, Hong Kong, Peoples R China
基金
中国国家自然科学基金;
关键词
Influenza A; Nucleoprotein; Rapid test; Dot-ELISA; H1N1; VIRUS; MONOCLONAL-ANTIBODIES; NUCLEOPROTEIN; SENSITIVITY; HUMANS;
D O I
10.1016/j.jviromet.2010.02.001
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:100 / 102
页数:3
相关论文
共 50 条
  • [1] Rapid Detection of Reassortment of Pandemic H1N1/2009 Influenza Virus
    Poon, Leo L. M.
    Mak, Polly W. Y.
    Li, Olive T. W.
    Chan, Kwok Hung
    Cheung, Chung Lam
    Ma, Edward S.
    Yen, Hui-Ling
    Vijaykrishna, Dhanasekaran
    Guan, Yi
    Peiris, J. S. Malik
    CLINICAL CHEMISTRY, 2010, 56 (08) : 1340 - 1344
  • [2] Rapid detection of reassortment of pandemic H1N1/2009 influenza virus
    Mak, Polly
    Li, Olive
    Vijaykrishna, Dhanasekaran
    Guan, Yi
    Peiris, Joseph Sriyal Malik
    Poon, Leo
    INFLUENZA AND OTHER RESPIRATORY VIRUSES, 2011, 5 : 132 - 134
  • [3] Detection Rate of Rapid Antigen Test for Pandemic Influenza A (H1N1 2009)
    Kim, Young Keun
    Kim, Hyo Youl
    Uh, Young
    Chun, Jin-Kyong
    INFECTION AND CHEMOTHERAPY, 2010, 42 (02): : 95 - 98
  • [4] Evaluation of QuickVue influenza A plus B rapid test for detection of pandemic influenza A/H1N1 2009
    Velasco, John Mark S.
    Montesa-Develos, Maribel L.
    Jarman, Richard G.
    Lopez, Ma. Nila B.
    Gibbons, Robert V.
    Valderama, Maria Theresa G.
    Yoon, In-Kyu
    JOURNAL OF CLINICAL VIROLOGY, 2010, 48 (02) : 120 - 122
  • [5] Rapid Influenza Antigen Test for Diagnosis of Pandemic (H1N1) 2009
    Louie, Janice K.
    Guevara, Hugo
    Boston, Erica
    Dahlke, Melissa
    Nevarez, Maria
    Kong, Tong
    Schechter, Robert
    Glaser, Carol A.
    Schnurr, David P.
    EMERGING INFECTIOUS DISEASES, 2010, 16 (05) : 824 - 826
  • [6] Performance of rapid-test kits for the detection of the pandemic influenza A/H1N1 virus
    Tsao, Kuo-Chien
    Kuo, Yung-Bin
    Huang, Chung-Guei
    Chau, Shao-Wen
    Chan, Err-Cheng
    JOURNAL OF VIROLOGICAL METHODS, 2011, 173 (02) : 387 - 389
  • [7] 2009 H1N1 Influenza Pandemic
    Woo, Teri Moser
    JOURNAL OF PEDIATRIC HEALTH CARE, 2010, 24 (04) : 258 - 266
  • [8] Development and Comparison of Molecular Assays for the Rapid Detection of the Pandemic Influenza A (H1N1) 2009 Virus
    Lam, Wai-Yip
    Leung, Ting-Fan
    Lee, Nelson
    Cheung, Jo L. K.
    Yeung, Apple C. M.
    Ho, Yolanda I. I.
    Chan, Rickjason C. W.
    Fung, Kitty S. C.
    Barr, Ian G.
    Hui, David S. C.
    Sung, Joseph J. Y.
    Chan, Paul K. S.
    JOURNAL OF MEDICAL VIROLOGY, 2010, 82 (04) : 675 - 683
  • [9] Evaluation of a rapid diagnostic test, NanoSign® Influenza A/B Antigen, for detection of the 2009 pandemic influenza A/H1N1 viruses
    Lee, Gyu-Cheol
    Jeon, Eun-Sung
    Kim, Won-Shik
    Le, Dung Tien
    Yoo, Jong-Ha
    Chong, Chom-Kyu
    VIROLOGY JOURNAL, 2010, 7
  • [10] Sensitivity Assessment of Rapid Influenza Diagnostic Tests for the Detection of the 2009 Pandemic Influenza A (H1N1) Virus in Clinical Specimens
    Chen Ming
    Xiao Wei
    Ai Biao
    He Han
    Liu Xuezheng
    Dong Hong
    Xiong Jun
    Xu Li
    LABMEDICINE, 2010, 41 (12): : 731 - 734