Cloning and sequencing of canine MAGE cDNA

被引：6

作者：

Ma, Z

Khatlani, TS

Ohno, K

Sasaki, K

Inokuma, H

Onishi, T

机构：

[1] Yamaguchi Univ, Fac Agr, Lab Vet Internal Med, Yamaguchi 7538515, Japan

[2] Univ Tokyo, Fac Agr, Lab Vet Internal Med, Tokyo 113, Japan

来源：

TISSUE ANTIGENS | 2000年 / 56卷 / 02期

关键词：

canine; melanoma antigen (MAGE); testis;

D O I：

10.1034/j.1399-0039.2000.560210.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Melanoma antigens (MAGE) are regarded to induce tumour-specific immune response and thought to be potential therapeutical agents for cancer immunotherapy. We hereby report the canine MAGE cDNA cloned from the testis of a beagle dog. Canine MAGE cDNA is 1455 base pair (bp) nucleotides in length, and contains an open reading frame (ORF) of 1137 bp nucleotides encoding a protein of 378 amino acids. The predicted amino acid sequence has 22-49% of homology with other MAGE proteins. mRNA transcripts of canine MAGE were detected only in the melanoma and testis and not in other normal tissues of adult dog by reverse transcriptase polymerase chain reaction (RT-PCR), indicating that the expression pattern of canine MAGE mRNA is similar to that of the MAGE family genes in tumor and normal tissues.

引用

页码：166 / 169

页数：4

共 14 条

[1]

Chaux P, 1999, J IMMUNOL, V163, P2928

[2] A new family of mouse genes homologous to the human MAGE genes [J].

De Plaen, E ;

De Backer, O ;

Arnaud, D ;

Bonjean, B ;

Chomez, P ;

Martelange, V ;

Avner, P ;

Baldacci, P ;

Babinet, C ;

Hwang, SY ;

Knowles, B ;

Boon, T .

GENOMICS, 1999, 55 (02) :176-184

[3] STRUCTURE, CHROMOSOMAL LOCATION, AND EXPRESSION PATTERN OF 3 MOUSE GENES HOMOLOGOUS TO THE HUMAN MAGE GENES [J].

DEBACKER, O ;

VERHEYDEN, AM ;

MARTIN, B ;

GODELAINE, D ;

DEPLAEN, E ;

BRASSEUR, R ;

AVNER, P ;

BOON, T .

GENOMICS, 1995, 28 (01) :74-83

[4] STRUCTURE, CHROMOSOMAL LOCALIZATION, AND EXPRESSION OF 12 GENES OF THE MAGE FAMILY [J].

DEPLAEN, E ;

ARDEN, K ;

TRAVERSARI, C ;

GAFORIO, JJ ;

SZIKORA, JP ;

DESMET, C ;

BRASSEUR, F ;

VANDERBRUGGEN, P ;

LETHE, B ;

LURQUIN, C ;

BRASSEUR, R ;

CHOMEZ, P ;

DEBACKER, O ;

CAVENEE, W ;

BOON, T .

IMMUNOGENETICS, 1994, 40 (05) :360-369

[5] Generation of specific antitumor reactivity by the stimulation of spleen cells from gastric cancer patients with MAGE-3 synthetic peptide [J].

Fujie, T ;

Tanaka, F ;

Tahara, K ;

Li, J ;

Tanaka, S ;

Mori, M ;

Ueo, H ;

Takesako, K ;

Akiyoshi, T .

CANCER IMMUNOLOGY IMMUNOTHERAPY, 1999, 48 (04) :189-194

[6] The potential of melanoma antigen expression in cancer therapy [J].

Gillespie, AM ;

Coleman, RE .

CANCER TREATMENT REVIEWS, 1999, 25 (04) :219-227

[7] Naturally occurring cancer in pet dogs: Important models for developing improved cancer therapy in humans [J].

Knapp, DW ;

Waters, DJ .

MOLECULAR MEDICINE TODAY, 1997, 3 (01) :8-11

[8]

Lucas S, 1998, CANCER RES, V58, P743

[9] Two members of the human MAGEB gene family located in Xp21.3 are expressed in tumors of various histological origins [J].

Lurquin, C ;

De Smet, C ;

Brasseur, F ;

Muscatelli, F ;

Martelange, V ;

De Plaen, E ;

Brasseur, R ;

Monaco, AP ;

Boon, T .

GENOMICS, 1997, 46 (03) :397-408

[10]

Marchand M, 1999, INT J CANCER, V80, P219, DOI 10.1002/(SICI)1097-0215(19990118)80:2<219::AID-IJC10>3.0.CO

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