Regulatory T-cells have a prominent role in the immune modulated vaccine response by specific oligosaccharides

被引：35
作者：
van't Land, Belinda [1 ,2 ]
Schijf, Marcel [2 ,3 ]
van Esch, Betty C. A. M. [2 ]
van Bergenhenegouwen, Jeroen [2 ]
Bastiaans, Jacqueline [2 ]
Schouten, Bastiaan
Boon, Louis [4 ]
Garssen, Johan [2 ]
机构：
[1] Univ Utrecht, Dept Pharmaceut Sci, Div Pharmacol & Pathophysiol, Utrecht Inst Pharmaceut Sci, NL-3508 TB Utrecht, Netherlands
[2] Danone Res Ctr Specialised Nutr, Wageningen, Netherlands
[3] Univ Med Ctr, Dept Pediat, Wilhelmina Childrens Hosp, Utrecht, Netherlands
[4] Bioceros, Utrecht, Netherlands
来源：
VACCINE | 2010年 / 28卷 / 35期
关键词：
Regulatory T-cells (Treg); PC61; Flu-vaccination; Non-digestible oligosaccharides; TRANSCRIPTION FACTOR; IN-VIVO; AUTOIMMUNE-DISEASE; CUTTING EDGE; INDUCTION; DEPLETION; INFLUENZA; MIXTURE; LIFE; BET;
D O I：
10.1016/j.vaccine.2010.06.046
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Regulatory T-cells are increasingly important in vaccine strategies. In a Flu-vaccination model the role of CD4(+)CD25(+)Foxp3(+) regulatory T-cells (Tregs) and the immune modulation by orally supplied prebiotic oligosaccharides consisting of scGOS/lcFOS/pAOS, were assessed using anti-CD25 (PC61) mediated depletion studies As expected, in C57BL/6J mice the Flu-vaccination resulted in significantly (p < 0 001) increased DTH responses when receiving scGOS/lcFOS/pAOS. In addition, increased T-bet expression of activated CD4(+) T-cells was detected compared to placebo. In vivo depletion of CD25(+) Tregs significantly (p<0.05) increased basal DTH responses, indicating the suppressive function of these CD25(+) Tregs normally present Surprisingly, in vivo Tregs depletion diminished scGOS/lcFOS/pAOS induced immune modulation completely to control levels (p<0.05). Although no difference in number, percentage or activation of Tregs could be determined after scGOS/lcFOS/pAOS supplementation, changes in Treg function still remains to be investigated In conclusion, CD25(+) Tregs have an important role in modulated Flu-vaccine responses induced by scGOS/lcFOS/pAOS. (C) 2010 Elsevier Ltd All rights reserved.
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页码：5711 / 5717
页数：7
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