Messenger RNA modifications: Form, distribution, and function

被引：500

作者：

Gilbert, Wendy V. ^{[1
]}

Bell, Tristan A. ^{[1
,2
]}

Schaening, Cassandra ^{[1
,3
]}

机构：

[1] MIT, Dept Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[2] MIT, Grad Program Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[3] MIT, Grad Program Computat & Syst Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

来源：

SCIENCE | 2016年 / 352卷 / 6292期

关键词：

SINGLE-NUCLEOTIDE-RESOLUTION; MAPPING REVEALS; NUCLEAR-RNA; WIDESPREAD OCCURRENCE; N-6-METHYLADENOSINE; METHYLATION; YEAST; PSEUDOURIDYLATION; N6-METHYLADENOSINE; IDENTIFICATION;

D O I：

10.1126/science.aad8711

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

RNA contains more than 100 distinct modifications that promote the functions of stable noncoding RNAs in translation and splicing. Recent technical advances have revealed widespread and sparse modification of messenger RNAs with N-6-methyladenosine (m(6)A), 5-methylcytosine (m(5)C), and pseudouridine (psi). Here we discuss the rapidly evolving understanding of the location, regulation, and function of these dynamic mRNA marks, collectively termed the epitranscriptome. We highlight differences among modifications and between species that could instruct ongoing efforts to understand how specific mRNA target sites are selected and how their modification is regulated. Diverse molecular consequences of individual m(6)A modifications are beginning to be revealed, but the effects of m(5)C and psi remain largely unknown. Future work linking molecular effects to organismal phenotypes will broaden our understanding of mRNA modifications as cell and developmental regulators.

引用

页码：1408 / 1412

页数：5

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