Inducible expression of the GLT-1 glutamate transporter in a CHO cell line selected for low endogenous glutamate uptake

被引：18

作者：

Levy, LM

Attwell, D

Hoover, F

Ash, JF

Bjorås, M

Danbolt, NC

机构：

[1] UCL, Dept Physiol, London WC1E 6BT, England

[2] Univ Oslo, Dept Anat, N-0317 Oslo, Norway

[3] Univ Oslo, Dept Neurophysiol, N-0317 Oslo, Norway

[4] Univ Utah, Dept Neurobiol & Anat, Salt Lake City, UT 84132 USA

[5] Natl Hosp Norway, Inst Med Microbiol, N-0027 Oslo, Norway

来源：

FEBS LETTERS | 1998年 / 422卷 / 03期

基金：

英国惠康基金;

关键词：

glutamate; glutamate transporter; inducible expression; CHO cell;

D O I：

10.1016/S0014-5793(98)00036-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inducible expression of the mammalian glial cell glutamate transporter GLT-1 has been established in a CHO cell line selected for low endogenous Na+-dependent glutamate uptake by [H-3]aspartate suicide selection, Culturing the cells in doxycycline-containing medium, to activate GLT-1 expression via the Tet-On system, increased uptake of the GLT-1 substrate D-aspartate 280-fold, and increased cell size, Applying glutamate to whole-cell clamped, doxycycline-treated cells evoked a transporter-mediated current with characteristics appropriate for GLT-1, This cell line provides a useful tool for further examination of the electrical, biochemical and pharmacological properties of GET-I, the most abundant glutamate transporter in the brain. (C) 1998 Federation of European Biochemical Societies.

引用

页码：339 / 342

页数：4

共 23 条

[21] STRUCTURE, EXPRESSION, AND FUNCTIONAL-ANALYSIS OF A NA+-DEPENDENT GLUTAMATE ASPARTATE TRANSPORTER FROM RAT-BRAIN [J].