Protective efficacy of rutin against acrylamide-induced oxidative stress, biochemical alterations and histopathological lesions in rats

被引:9
作者
Uthra, Chhavi [1 ]
Reshi, Mohd Salim [2 ]
Jaswal, Amita [1 ]
Yadav, Deepa [1 ]
Shrivastava, Sadhana [1 ]
Sinha, Neelu [1 ]
Shukla, Sangeeta [1 ]
机构
[1] Jiwaji Univ, Reprod Biol & Toxicol Lab, UNESCO Trac Element Satellite Ctr, Sch Studies Zool, Gwalior 474011, Madhya Pradesh, India
[2] Baba Ghulam Shah Badshah Univ, Toxicol & Pharmacol Lab, Dept Zool, Sch Biosci & Biotechnol, Rajouri 185234, Jammu & Kashmir, India
关键词
rutin; acrylamide; antioxidant; oxidative stress; liver; kidney; brain; INDUCED TOXICITY; ANTIOXIDANTS; INJURY; HEPATOTOXICITY; CANCER; ASSAY; MICE;
D O I
10.1093/toxres/tfab125
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Acrylamide is a well-known neurotoxicant and carcinogen. Apart from industrial exposure, acrylamide is also found in different food products. The present study deals with in vivo experiment to test the protective effect of rutin against acrylamide induced toxicity in rats. The study was carried out on female rats with exposure of acrylamide at the dose of 38.27 mg/kg body weight, orally for 10 days followed by the therapy of rutin (05, 10, 20 and 40 mg/kg orally), for three consecutive days. All animals were sacrificed after 24 h of last treatment and various biochemical parameters in blood and tissue were investigated. Histopathology of liver, kidney and brain was also done. On administration of acrylamide for 10 days, neurotoxicity was observed in terms of decreased acetylcholinesterase activity and oxidative stress was observed in terms of increased lipid peroxidation, declined level of reduced glutathione, antioxidant enzymes (superoxide dismutase and catalase) in liver, kidney and brain. Acrylamide exposure increased the activities of serum transaminases, lipid profile, bilirubin, urea, uric acid and creatinine in serum indicating damage. Our experimental results conclude that rutin showed remarkable protection against oxidative DNA damage induced by acrylamide, which may be due to its antioxidant potential.
引用
收藏
页码:215 / 225
页数:11
相关论文
共 66 条
[1]   Protective effects of thymoquinone against acrylamide-induced liver, kidney and brain oxidative damage in rats [J].
Abdel-Daim, Mohamed M. ;
El-Ela, Fatma I. Abo ;
Alshahrani, Fatima K. ;
Bin-Jumah, May ;
Al-Zharani, Mohammed ;
Almutairi, Bader ;
Alyousif, Mohamed S. ;
Bungau, Simona ;
Aleya, Lotfi ;
Alkahtani, Saad .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2020, 27 (30) :37709-37717
[2]   Trigonella foenum-graecum ameliorates acrylamide-induced toxicity in rats: Roles of oxidative stress, proinflammatory cytokines, and DNA damage [J].
Abdel-Daim, Mohamed M. ;
Abd Eldaim, Mabrouk A. ;
Hassan, Abeer G. A. .
BIOCHEMISTRY AND CELL BIOLOGY, 2015, 93 (03) :192-198
[3]  
Aebi H., 1984, METHOD ENZYMOL, P121
[4]  
Agrawal PK., 2021, INHIBITOR NAT PROD C, V16
[5]   The protective effects of 18β-glycyrrhetinic acid against acrylamide-induced cellular damage in diabetic rats [J].
Alanazi, Ibtesam S. ;
Emam, Mohamed ;
Elsabagh, Mabrouk ;
Alkahtani, Saad ;
Abdel-Daim, Mohamed M. .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2021, 28 (41) :58322-58330
[6]   Prenatal and perinatal acrylamide disrupts the development of cerebellum in rat: Biochemical and morphological studies [J].
Allam, A. ;
El-Gharee, A. A. ;
Abdul-Hamid, M. ;
Baikry, A. ;
Sabri, M. I. .
TOXICOLOGY AND INDUSTRIAL HEALTH, 2011, 27 (04) :291-306
[7]  
[Anonymous], 1994, STAT METHODOL
[8]  
[Anonymous], 2017, REPORTBUYER 0309
[9]  
Banagozar Mohammadi Ali, 2015, IRAN J TOXICOL, V9, P1284
[10]   Is the dietary acrylamide exposure in Chile a public health problem? [J].
Barrios-Rodriguez, Yeison Fernando ;
Pedreschi, Franco ;
Rosowski, Jaime ;
Gomez, Juan Pablo ;
Figari, Nicole ;
Castillo, Oscar ;
Mariotti Celis, Maria Salome .
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2021, 38 (07) :1126-1135