Osteoclast differentiation factor in human osteosarcoma cell line

被引：29
作者：
Kinpara, K [1 ]
Mogi, M [1 ]
Kuzushima, M [1 ]
Togari, A [1 ]
机构：
[1] Aichi Gakuin Univ, Sch Dent, Dept Pharmacol, Nagoya, Aichi 4648650, Japan
来源：
JOURNAL OF IMMUNOASSAY | 2000年 / 21卷 / 04期
关键词：
ODF; OCIF; ELISA; sarcoma; MC3T3-E1;
D O I：
10.1080/01971520009349540
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for human osteoclast differentiation factor (ODF/RANKL/OPGL/TRANCE) utilizing a polyclonal antibody that recognizes both human soluble ODF and mouse ODF in combination with a osteoclasogenesis inhibitory factor (OCIF/OPG) was developed. We can quantify the ODF level in not only human ODF (detection limit: 0.05 ng/ml), but also mouse ODF by virtue of cross-reactivity; Employing this assay system, we demonstrated that ODF is constitutively present as a membrane-bound form in both the human osteosarcoma cell lines, MG-63, HOS and SaOS-2, and the mouse osteoblastic cell line MC3T3-E1.
引用
收藏
页码：327 / 340
页数：14
相关论文
共 18 条
[1]   The ligand for osteoprotegerin (OPGL) directly activates mature osteoclasts [J].
Burgess, TL ;
Qian, YX ;
Kaufman, S ;
Ring, BD ;
Van, G ;
Capparelli, C ;
Kelley, M ;
Hsu, HL ;
Boyle, WJ ;
Dunstan, CR ;
Hu, S ;
Lacey, DL .
JOURNAL OF CELL BIOLOGY, 1999, 145 (03) :527-538
[2]   Interactions between cancer and bone marrow cells induce osteoclast differentiation factor expression and osteoclast-like cell formation in vitro [J].
Chikatsu, N ;
Takeuchi, Y ;
Tamura, Y ;
Fukumoto, S ;
Yano, K ;
Tsuda, E ;
Ogata, E ;
Fujita, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 267 (02) :632-637
[3]   TRANCE is necessary and sufficient for osteoblast-mediated activation of bone resorption in osteoclasts [J].
Fuller, K ;
Wong, B ;
Fox, S ;
Choi, YW ;
Chambers, TJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (05) :997-1001
[4]   Osteotropic agents regulate the expression of osteoclast differentiation factor and osteoprotegerin in osteoblastic stromal cells. [J].
Horwood, NJ ;
Elliott, J ;
Martin, TJ ;
Gillespie, MT .
ENDOCRINOLOGY, 1998, 139 (11) :4743-4746
[5]   Activated T cells regulate bone loss and joint destruction in adjuvant arthritis through osteoprotegerin ligand [J].
Kong, YY ;
Feïge, U ;
Sarosi, I ;
Bolon, B ;
Tafuri, A ;
Morony, S ;
Capparelli, C ;
Li, J ;
Elliott, R ;
McCabe, S ;
Wong, T ;
Campagnuolo, G ;
Moran, E ;
Bogoch, ER ;
Van, G ;
Nguyen, LT ;
Ohashi, PS ;
Lacey, DL ;
Fish, E ;
Boyle, WJ ;
Penninger, JM .
NATURE, 1999, 402 (6759) :304-309
[6]   OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis [J].
Kong, YY ;
Yoshida, H ;
Sarosi, I ;
Tan, HL ;
Timms, E ;
Capparelli, C ;
Morony, S ;
Oliveira-dos-Santos, AJ ;
Van, G ;
Itie, A ;
Khoo, W ;
Wakeham, A ;
Dunstan, CR ;
Lacey, DL ;
Mak, TW ;
Boyle, WJ ;
Penninger, JM .
NATURE, 1999, 397 (6717) :315-323
[7]   IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis [J].
Kotake, S ;
Udagawa, N ;
Takahashi, N ;
Matsuzaki, K ;
Itoh, K ;
Ishiyama, S ;
Saito, S ;
Inoue, K ;
Kamatani, N ;
Gillespie, MT ;
Martin, TJ ;
Suda, T .
JOURNAL OF CLINICAL INVESTIGATION, 1999, 103 (09) :1345-1352
[8]   Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation [J].
Lacey, DL ;
Timms, E ;
Tan, HL ;
Kelley, MJ ;
Dunstan, CR ;
Burgess, T ;
Elliott, R ;
Colombero, A ;
Elliott, G ;
Scully, S ;
Hsu, H ;
Sullivan, J ;
Hawkins, N ;
Davy, E ;
Capparelli, C ;
Eli, A ;
Qian, YX ;
Kaufman, S ;
Sarosi, I ;
Shalhoub, V ;
Senaldi, G ;
Guo, J ;
Delaney, J ;
Boyle, WJ .
CELL, 1998, 93 (02) :165-176
[9]   TRANSFORMING GROWTH-FACTOR-ALPHA IN HUMAN SUBMANDIBULAR-GLAND AND SALIVA [J].
MOGI, M ;
INAGAKI, H ;
KOJIMA, K ;
MINAMI, M ;
HARADA, M .
JOURNAL OF IMMUNOASSAY, 1995, 16 (04) :379-394
[10]   DETECTION OF INACTIVE OR LESS ACTIVE FORMS OF TYROSINE-HYDROXYLASE IN HUMAN ADRENALS BY A SANDWICH ENZYME-IMMUNOASSAY [J].
MOGI, M ;
KOJIMA, K ;
NAGATSU, T .
ANALYTICAL BIOCHEMISTRY, 1984, 138 (01) :125-132
12