Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting

被引：11
|
作者：
Zhukov, Alex A. [1 ]
Pritchard, Robyn H. [2 ]
Withers, Mick J. [2 ]
Hailes, Tony [2 ]
Gold, Richard D. [2 ]
Hayes, Calum [2 ]
la Cour, Mette F. [2 ]
Hussein, Fred [2 ]
Rogers, Salman Samson [1 ]
机构：
[1] Cellular Highways Ltd, Melbourn Sci Pk, Melbourn SG8 6EE, Cambs, England
[2] TTP PLC, Melbourn Sci Pk, Melbourn SG8 6EE, Cambs, England
来源：
MICROMACHINES | 2021年 / 12卷 / 04期
关键词：
microfluidics; cell sorting; high-throughput; VACS; cytometry; T-CELLS; FLOW; SORTER; PURIFICATION; SEPARATION;
D O I：
10.3390/mi12040389
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We demonstrate extremely high-throughput microfluidic cell sorting by making a parallel version of the vortex-actuated cell sorter (VACS). The set-up includes a parallel microfluidic sorter chip and parallel cytometry instrumentation: optics, electronics and control software. The result is capable of sorting lymphocyte-sized particles at 16 times the rate of our single-stream VACS devices, and approximately 10 times the rate of commercial cell sorters for an equivalent procedure. We believe this opens the potential to scale cell sorting for applications requiring the processing of much greater cell numbers than currently possible with conventional cell sorting.
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页数：12
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