Mesoscopic Epifluorescence Tomography: Reconstruction of superficial and deep fluorescence in highly-scattering media

被引：20

作者：

Bjoern, Saskia ^{[1
]}

Ntziachristos, Vasilis ^{[1
]}

Schulz, Ralf ^{[1
]}

机构：

[1] Helmholtz Zentrum Munchen, IBMI, D-85764 Neuherberg, Germany

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 08期

关键词：

LIGHT; TRANSPORT;

D O I：

10.1364/OE.18.008422

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Mesoscopic Epifluorescence Tomography (MEFT) is a technique derived from Laminar Optical Tomography (LOT), determining fluorescence biodistribution by tomographic means in reflectance geometry. A pencil beam is scanned over the region of interest to excite fluorophores hidden within the tissue, while a CCD camera acquires images of reflected fluorescence emissions. This configuration is advantageous whenever transillumination of the specimen is not feasible, e. g., in the presence of skin chambers or when using wavelengths in the visible range where absorption is high. We present simulation and phantom studies recovering deep GFP-like fluorescence in highly scattering and strongly absorbing media with a penetration depth up to 10mm. (C) 2010 Optical Society of America

引用

页码：8422 / 8429

页数：8

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