Identification and Characterization of Molten Globule-Like State of Hen Egg-White Lysozyme in Presence of Salts Under Alkaline Conditions

被引：19

作者：

Ansari, M. A. ^{[1
]}

Zubair, S. ^{[2
]}

Atif, S. M. ^{[1
]}

Kashif, M. ^{[1
]}

Khan, N. ^{[1
]}

Rehan, M. ^{[1
]}

Anwar, T. ^{[1
]}

Iqbal, A. ^{[3
]}

Owais, M. ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Aligarh 202002, Uttar Pradesh, India

[2] Aligarh Muslim Univ, Womens Coll, Aligarh 202002, Uttar Pradesh, India

[3] Aligarh Muslim Univ, Dept Agr Microbiol, Aligarh 202002, Uttar Pradesh, India

来源：

PROTEIN AND PEPTIDE LETTERS | 2010年 / 17卷 / 01期

关键词：

Lysozyme; secondary structure; molten globule; ELECTROSTATIC INTERACTIONS; INTERMEDIATE STATE; STRUCTURAL-CHARACTERIZATION; CIRCULAR-DICHROISM; ALPHA-LACTALBUMIN; PROTEIN-STRUCTURE; ESCHERICHIA-COLI; CYTOCHROME-C; BINDING; COOPERATIVITY;

D O I：

10.2174/092986610789909502

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, we elucidated the effect of potassium salts on alkali denatured hen egg white lysozyme (EC 3.2.1.17) using intrinsic/extrinsic fluorescence as well circular dichroism (CD) spectroscopic methods. Intrinsic fluorescence studies revealed that various potassium salts mediate stabilization of lysozyme against alkali denaturation. Far and near UV CD spectrum studies, showed that 2M KCl induced appreciable amount of secondary structure with minimum tertiary contacts in lysozyme at pH 12.6. Acrylamide quenching studies suggest that at pH 12.6, the presence of 2M KCl causes reduced accessibility of the quencher to tryptophan residues of the protein presumably because of its compact conformation. In summary, the results of present study suggest that lysozyme attains a compact folded intermediate with molten globule like characteristics at alkaline pH in presence of potassium chloride.

引用

页码：11 / 17

页数：7

共 58 条

[1] 8-anilino-1-naphthalene sulfonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions [J].