Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

被引：786

作者：

Adewumi, Oluseun

Aflatoonian, Behrouz

Ahrlund-Richter, Lars

Amit, Michal

Andrews, Peter W. ^{[1
]}

Beighton, Gemma

Bello, Paul A.

Benvenisty, Nissim

Berry, Lorraine S.

Bevan, Simon

Blum, Barak

Brooking, Justin

Chen, Kevin G.

Choo, Andre B. H.

Churchill, Gary A.

Corbel, Marie

Damjanov, Ivan

Draper, Jon S.

Dvorak, Petr

Emanuelsson, Katarina

Fleck, Roland A.

Ford, Angela

Gertow, Karin

Gertsenstein, Marina

Gokhale, Paul J.

Hamilton, Rebecca S.

Hampl, Ales

Healy, Lyn E.

Hovatta, Outi

Hyllner, Johan

Imreh, Marta P.

Itskovitz-Eldor, Joseph

Jackson, Jamie

Johnson, Jacqueline L.

Jones, Mark

Kee, Kehkooi

King, Benjamin L.

Knowles, Barbara B.

Lako, Majlinda

Lebrin, Franck

Mallon, Barbara S.

Manning, Daisy

Mayshar, Yoav

Mckay, Ronald D. G.

Michalska, Anna E.

Mikkola, Milla

Mileikovsky, Masha

Minger, Stephen L.

Moore, Harry D.

Mummery, Christine L.

机构：

[1] Univ Sheffield, Dept Biomed Sci, Ctr Stem Cell Biol, Sheffield S10 2TN, S Yorkshire, England

[2] Natl Inst Biol Stand & Controls, UK Stem Cell Bank, Div Cell Biol & Imaging, S Mimms EN6 3QG, Herts, England

[3] Univ Sheffield, Ctr Stem Cell Biol, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England

[4] Karolinska Inst, Dept Lab Med, Unit Mol Embryol, S-14157 Huddinge, Sweden

[5] Technion Israel Inst Technol, Rambam Med Ctr, IL-31096 Haifa, Israel

[6] St James Univ Hosp, Canc Res UK Genotyping Facil, Leeds LS9 3LP, W Yorkshire, England

[7] Geneservice Ltd, Cambridge CB3 0FE, England

[8] Monash Univ, Stem Cell Sci Ltd, Clayton, Vic 3800, Australia

[9] Hebrew Univ Jerusalem, Inst Life Sci, Dept Genet, IL-91904 Jerusalem, Israel

[10] Thermo Fisher Sci, Matrix Technol, Handforth SK9 3LP, England

[11] NIH, NIH Stem Cell Unit, Bethesda, MD 20892 USA

[12] Bioproc Technol Inst, Singapore 138668, Singapore

[13] Jackson Lab, Bar Harbor, ME 04609 USA

[14] Univ Cambridge, Addenbrookes Hosp, Dept Surg, Cambridge CB2 2XY, England

[15] Univ Kansas, Sch Med, Dept Pathol, Kansas City, KS 66160 USA

[16] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[17] Masaryk Univ, Fac Med, Dept Biol, Brno, Czech Republic

[18] Acad Sci Czech Republ, Inst Expt Med, Dept Mol Embryol, Brno, Czech Republic

[19] Cellartis AB, S-41346 Gothenburg, Sweden

[20] Monash Univ, MISCL, Clayton, Vic 3800, Australia

[21] Karolinska Univ Hosp, Karolinska Inst, Dept Clin Sci Intervent & Technol, Div Obstet Gynecol, S-14186 Huddinge, Sweden

[22] Karolinska Inst, Dept Lab Med, Clin Res Ctr, S-14157 Huddinge, Sweden

[23] Uppsala Univ, Dept Genet & Dev, SE-7523236 Uppsala, Sweden

[24] Stanford Univ, Ctr Human Embryon Stem Cell Res & Educ, Inst Stem Cell Biol & Regenerat Med, Dept Obstet & Gynecol, Palo Alto, CA 94304 USA

[25] Jackson Lab, Bar Harbor, ME 04609 USA

[26] Inst Mol Biophys, Bar Harbor, ME 04609 USA

[27] Newcastle Univ, Inst Human Genet, Int Ctr Life, Newcastle Upon Tyne NE1 3BZ, Tyne & Wear, England

[28] Hubrecht Lab, NL-3584 CT Utrecht, Netherlands

[29] Univ Paris 06, INSERM, UR 525, Paris, France

[30] WiCell Res Inst, Madison, WI 53707 USA

[31] WiCell Res Inst, St Louis, MO 63131 USA

[32] Monash Univ, Monash Inst Med Res, Lab Embryon Stem Cell Biol, Clayton, Vic 3800, Australia

[33] Australian Stem Cell Ctr, Clayton, Vic 3800, Australia

[34] Univ Helsinki, Biomedicum Stem Cell Ctr, Biomedicum Helsinki, FIN-00014 Helsinki, Finland

[35] Kings Coll London, Wolfson Ctr Age Related Dis, London SE1 1UL, England

[36] Kyoto Univ, Inst Frontier Med Sci, Kyoto 6068507, Japan

[37] UCL, Univ Coll London, Regenerat Med Bioproc Unit, Adv Ctr Biochem Engn,Dept Biochem Engn, London WC1E 7JE, England

[38] Monash Univ, Monash Inst Med Res, Ctr Reprod & Dev, Clayton, Vic 3800, Australia

[39] Univ So Calif, Keck Sch Med, Ctr Stem Cell & Regenerat Med, CO Zilkha Neurogenet Inst, Los Angeles, CA 90089 USA

[40] Hadassah Univ, Med Ctr, Hadassah Human Embryon Stem Cell Res Ctr, Goldyne Savad Inst Gene Therapy, IL-91120 Jerusalem, Israel

[41] Hadassah Univ, Med Ctr, Dept Obstet & Gynecol, IL-91120 Jerusalem, Israel

[42] Novocell, Athens, GA 30606 USA

[43] Hosp Sick Children, Dev Biol Program, Toronto, ON M5G 1L7, Canada

[44] Hosp Sick Children, Dev Biol Program, Toronto, ON M5G 1X8, Canada

[45] Lund Univ, Stem Cell Ctr, SE-22184 Lund, Sweden

[46] Ctr Invest Principe Felipe, Valencia, Spain

来源：

NATURE BIOTECHNOLOGY | 2007年 / 25卷 / 07期

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会;

关键词：

D O I：

10.1038/nbt1318

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

引用

页码：803 / 816

页数：14

共 77 条

[1] Unique gene expression signatures of independently-derived human embryonic stem cell lines
Abeyta, MJ
Clark, AT
Rodriguez, RT
Bodnar, MS
Pera, RAR
Firpo, MT
[J]. HUMAN MOLECULAR GENETICS, 2004, 13 (06) : 601 - 608
[2] Differences between human embryonic stem cell lines
Allegrucci, C.
Young, L. E.
[J]. HUMAN REPRODUCTION UPDATE, 2007, 13 (02) : 103 - 120
[3] Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture
Amit, M
Carpenter, MK
Inokuma, MS
Chiu, CP
Harris, CP
Waknitz, MA
Itskovitz-Eldor, J
Thomson, JA
[J]. DEVELOPMENTAL BIOLOGY, 2000, 227 (02) : 271 - 278
[4] CELL-SURFACE ANTIGENS OF A CLONAL HUMAN EMBRYONAL CARCINOMA CELL-LINE - MORPHOLOGICAL AND ANTIGENIC DIFFERENTIATION IN CULTURE
ANDREWS, PW
GOODFELLOW, PN
SHEVINSKY, LH
BRONSON, DL
KNOWLES, BB
[J]. INTERNATIONAL JOURNAL OF CANCER, 1982, 29 (05) : 523 - 531
[5] RETINOIC ACID INDUCES NEURONAL DIFFERENTIATION OF A CLONED HUMAN EMBRYONAL CARCINOMA CELL-LINE INVITRO
ANDREWS, PW
[J]. DEVELOPMENTAL BIOLOGY, 1984, 103 (02) : 285 - 293
[6] Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: Opposite sides of the same coin
Andrews, PW
Matin, MM
Bahrami, AR
Damjanov, I
Gokhale, P
Draper, JS
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 2005, 33 : 1526 - 1530
[7] Andrews PW, 2005, NAT BIOTECHNOL, V23, P795, DOI 10.1038/nbt0705-798
[8] From teratocarcinomas to embryonic stem cells
Andrews, PW
[J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2002, 357 (1420) : 405 - 417
[9] 2 MONOCLONAL-ANTIBODIES RECOGNIZING DETERMINANTS ON HUMAN EMBRYONAL CARCINOMA-CELLS REACT SPECIFICALLY WITH THE LIVER ISOZYME OF HUMAN ALKALINE-PHOSPHATASE
ANDREWS, PW
MEYER, LJ
BEDNARZ, KL
HARRIS, H
[J]. HYBRIDOMA, 1984, 3 (01): : 33 - 39
[10] ANDREWS PW, 1987, CANCER RES, V47, P740

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