Molecular Characterization of Tandem Repeat Protein 36 Gene of Ehrlichia canis Detected in Naturally Infected Dogs from Peru

被引：12

作者：

Geiger, Joseph ^{[1
]}

Morton, Bridget A. ^{[2
,3
]}

Vasconcelos, Elton Jose Rosas ^{[2
]}

Tngrian, Maryam ^{[4
]}

Kachani, Malika ^{[2
]}

Barron, Eduardo A. ^{[5
,11
]}

Gavidia, Cesar M. ^{[5
,12
]}

Gilman, Robert H. ^{[6
,7
,8
,13
]}

Angulo, Noelia P. ^{[5
,14
]}

Lerner, Richard ^{[9
,15
]}

Scott, Tamerin ^{[10
]}

Mirrashed, N. Hannah ^{[2
]}

Oakley, Brian ^{[2
]}

Diniz, Pedro Paulo V. P. ^{[2
]}

机构：

[1] Western Univ Hlth Sci, Grad Coll Biomed Sci, Pomona, CA USA

[2] Western Univ Hlth Sci, Coll Vet Med, 309 E Second St, Pomona, CA 91766 USA

[3] Affiliated Vet Specialists, Orange Pk, FL USA

[4] Calif State Polytech Univ Pomona, Coll Agr, Pomona, CA 91768 USA

[5] Univ Nacl Mayor San Marcos, Lab Med Vet Prevent, Fac Med Vet, Lima, Peru

[6] Johns Hopkins Univ, Dept Int Hlth, Bloomberg Sch Publ Hlth, Baltimore, MD USA

[7] Univ Peruana Cayetano Heredia, Lab Invest Enfermedades Infecciosas, Dept Microbiol, Lima, Peru

[8] Asociac Benefica Proyectos Informat Salud Med & A, Lima, Peru

[9] Pan Amer Zoonot Res & Prevent PAZ, Framingham, MA USA

[10] Los Angeles Cty Dept Publ Hlth, Los Angeles, CA USA

[11] Coll Vet Med, Lima, Peru

[12] Natl Univ San Marcos, Coll Vet Med, Lima, Peru

[13] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Int Hlth, Baltimore, MD USA

[14] Univ Peruana Cayetano Heredia, Lab Invest Enfermedades Infecciosas, Lima, Peru

[15] Pan Amer Zoonot Res & Prevent, Framingham, MA USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2018年 / 99卷 / 02期

关键词：

MULTIPLE SEQUENCE ALIGNMENT; TICK; ANAPLASMA; GENOTYPE; DISEASES; STRAIN; IDENTIFICATION; BARTONELLA; PATHOGENS; FEVER;

D O I：

10.4269/ajtmh.17-0776

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Ehrlichia spp. are emerging infectious pathogens, especially in the Americas. Although Ehrlichia canis is primarily a parasite of dogs, polymerase chain reaction-confirmed human infections have been reported from Mexico, Venezuela, and Costa Rica. This study reports the presence of E. canis DNA in 13.7% of 205 dogs from urban areas in Peru and of those, five were analyzed for phylogenetic variation using the Tandem Repeat Protein 36 ( TRP36) gene. The use of the TRP36 gene for such analysis was validated against 16S rRNA and heat shock protein genes using Shannon's entropy bioinformatic approach. When compared with other E. canis strains previously reported, three unique and novel E. canis strains were detected. In addition, the TRP36 amino acid tandem repeat sequences of the Peruvian strains share close similarity to an E. canis strain detected from four human blood bank samples in Costa Rica. This study reports for the first time domestic dogs infected with E. canis strains closely related to a zoonotic strain, which may be of public health concern as dogs can be chronically infected with this pathogen.

引用

页码：297 / 302

页数：6

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