3D-PRINTING-ASSISTED FABRICATION OF CHITOSAN SCAFFOLDS FROM DIFFERENT SOURCES AND CROSS-LINKERS FOR DENTAL TISSUE ENGINEERING

被引：16

作者：

EzEldeen, M. ^{[1
,2
,3
,4
]}

Loos, J. ^{[5
]}

Nejad, Z. Mousavi ^{[1
,2
,6
]}

Cristaldi, M. ^{[1
,2
,7
]}

Murgia, D. ^{[1
,2
,7
]}

Braem, A. ^{[5
]}

Jacobs, R. ^{[1
,2
,8
]}

机构：

[1] Katholieke Univ Leuven, Dept Imaging & Pathol, Fac Med, OMFS IMPATH Res Grp, Kapucijnenvoer 33, B-3000 Leuven, Belgium

[2] Univ Hosp Leuven, Oral & Maxillofacial Surg, Kapucijnenvoer 33, B-3000 Leuven, Belgium

[3] Katholieke Univ Leuven, Dept Oral Hlth Sci, Kapucijnenvoer 33, B-3000 Leuven, Belgium

[4] Univ Hosp Leuven, Paediat Dent & Special Dent Care, Kapucijnenvoer 33, B-3000 Leuven, Belgium

[5] Katholieke Univ Leuven, Dept Mat Engn, Biomat & Tissue Engn Res Grp, Kasteelpk Arenberg 44, B-3001 Leuven, Belgium

[6] Mat & Energy Res Ctr, Biomat Res Grp, Dept Nanotechnol & Adv Mat, POB 31787-316, Karaj, Alborz, Iran

[7] Univ Palermo, Dept Surg Oncol & Oral Sci, Via Liborio Giuffre 5, I-90127 Palermo, Italy

[8] Karolinska Inst, Dept Dent Med, Stockholm, Sweden

来源：

EUROPEAN CELLS & MATERIALS | 2021年 / 40卷

关键词：

Chitosan; fungal chitosan; dental pulp stem cells; ENRICHED FIBRIN HYDROGEL; GLOBAL BURDEN; GENIPIN; LINKING; BIOMATERIALS; DESIGN; CELLS;

D O I：

10.22203/eCM.v041a31

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering. Chitosan-based scaffolds were prepared by the emulsion freeze-drying technique. Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure. Chemical composition and cross-linking were evaluated by Fourier transform infrared attenuated total reflectance spectroscopy. Compression tests were performed to evaluate scaffold mechanical properties. Scaffold degradation was evaluated by gravimetric method and SEM. Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction. Finally, human dental pulp stem cells (hDPSCs) viability was evaluated. The fabrication method used was successful in producing scaffolds with organised porosity. Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response. Chitosan-genipin (CS-GEN) scaffolds had the largest pore diameter, while the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds had the smallest. Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21. Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability. Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.

引用

页码：485 / 501

页数：17

共 64 条

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