A method to estimate acetylcholinesterase-active sites and turnover in insects

被引：28

作者：

Charpentier, A ^{[1
]}

Menozzi, P ^{[1
]}

Marcel, V ^{[1
]}

Villatte, F ^{[1
]}

Fournier, D ^{[1
]}

机构：

[1] Univ Toulouse 3, LSPCMIB, Grp Biochim Prot, F-31062 Toulouse, France

来源：

ANALYTICAL BIOCHEMISTRY | 2000年 / 285卷 / 01期

关键词：

acetylcholinesterase; titration; Drosophila; insecticide; resistance; overexpression;

D O I：

10.1006/abio.2000.4738

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy)-1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific. (C) 2000 Academic Press.

引用

页码：76 / 81

页数：6

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