Isolation of Leptospira interrogans Hardjoprajitno from vaginal fluid of a clinically healthy ewe suggests potential for venereal transmission

被引：36

作者：

Director, A. ^{[1
]}

Penna, B. ^{[1
]}

Hamond, C. ^{[1
]}

Loureiro, A. P. ^{[1
]}

Martins, G. ^{[1
]}

Medeiros, M. A. ^{[2
]}

Lilenbaum, W. ^{[1
]}

机构：

[1] Univ Fed Fluminense, Vet Bacteriol Lab, Dept Microbiol & Parasitol, BR-24210130 Niteroi, RJ, Brazil

[2] Brazilian Minist Hlth, Recombinant Technol Lab, Oswaldo Cruz Fdn, BR-21040360 Rio De Janeiro, RJ, Brazil

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2014年 / 63卷

关键词：

SEROVAR HARDJO; SHEEP; PCR; TRACTS; SEMEN; SPP; COWS;

D O I：

10.1099/jmm.0.065466-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A total of 15 adult ewes from one flock known to be seroreactive for leptospirosis was studied. Urine and vaginal fluid were collected from each animal to test for the presence of leptospires using bacterial culture and conventional PCR methods. One pure culture of Leptospira sp. was obtained from the vaginal fluid sample of a non-pregnant ewe. The isolate was characterized by DNA sequencing of the rrs and secY genes, variable-number of tandem-repeats (VNTR) analysis and serogrouping, and the isolate was typed as Leptospira interrogans serogroup Sejroe serovar Hardjo type Hardjoprajitno. This report indicates the presence of viable Leptospira in the vaginal fluid of a ewe, suggesting the potential for venereal transmission of leptospires in sheep.

引用

页码：1234 / 1236

页数：3

共 14 条

[1]

Cerri D, 1996, MICROBIOLOGICA, V19, P175

[2] Presence of antigen and antibodies in serum and genital discharges of cows from dairy herds naturally infected with Leptospira interrogans serovar hardjo [J].