Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus)

被引：43

作者：

Jezkova, Jana ^{[1
]}

Limpouchova, Zlata ^{[2
]}

Prediger, Jitka ^{[1
]}

Holubova, Nikola ^{[1
,2
]}

Sak, Bohumil ^{[2
]}

Konecny, Roman ^{[1
]}

Kvetonova, Dana ^{[2
]}

Hlaskova, Lenka ^{[2
]}

Rost, Michael ^{[1
]}

McEvoy, John ^{[3
]}

Rajsky, Dusan ^{[4
]}

Feng, Yaoyu ^{[5
,6
]}

Kvac, Martin ^{[1
,2
]}

机构：

[1] Univ South Bohemia Ceske Budejovice, Fac Agr, Studentska 1668, Ceske Budejovice 37005, Czech Republic

[2] Czech Acad Sci, Inst Parasitol, Biol Ctr, Branisovska 31, Ceske Budejovice 37005, Czech Republic

[3] North Dakota State Univ, Microbiol Sci Dept, 1523 Centennial Blvd,Van Es Hall, Fargo, ND 58102 USA

[4] Tech Univ Zvolen, Fac Forestry, Zvolen 96001, Slovakia

[5] South China Agr Univ, Coll Vet Med, Key Lab Zoonosis, Minist Agr, Guangzhou 510642, Peoples R China

[6] Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China

来源：

MICROORGANISMS | 2021年 / 9卷 / 04期

基金：

中国国家自然科学基金;

关键词：

adaptation; prevalence; biology; course of infection; infectivity; oocyst size; phylogeny; parasite; MOLECULAR CHARACTERIZATION; DISCONTINUOUS SUCROSE; GIARDIA-DUODENALIS; WASTE-WATER; LIFE-CYCLE; PARVUM; PREVALENCE; SPP; CATTLE; PARASITES;

D O I：

10.3390/microorganisms9040813

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cryptosporidium spp., common parasites of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutrias (Myocastor coypus). A total of 150 faecal samples of feral nutria were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci. Molecular analyses revealed the presence of C. parvum (n = 1), C. ubiquitum subtype family XIId (n = 5) and Cryptosporidium myocastoris n. sp. XXIIa (n = 2), and XXIIb (n = 3). Only nutrias positive for C. myocastoris shed microscopically detectable oocysts, which measured 4.8-5.2 x 4.7-5.0 mu m, and oocysts were infectious for experimentally infected nutrias with a prepatent period of 5-6 days, although not for mice, gerbils, or chickens. The infection was localised in jejunum and ileum without observable macroscopic changes. The microvilli adjacent to attached stages responded by elongating. Clinical signs were not observed in naturally or experimentally infected nutrias. Phylogenetic analyses at SSU, actin, and HSP70 loci demonstrated that C. myocastoris n. sp. is distinct from other valid Cryptosporidium species.

引用

页数：24

共 118 条

[41] Distribution of Cryptosporidium genotypes in storm event water samples from three watersheds in New York [J].