Paraflagellar rod is vital for trypanosome motility

被引：166

作者：

Bastin, P ^{[1
]}

Sherwin, T ^{[1
]}

Gull, K ^{[1
]}

机构：

[1] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England

来源：

NATURE | 1998年 / 391卷 / 6667期

关键词：

D O I：

10.1038/35300

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

African trypanosomes are protozoan parasites that cause sleeping sickness in man. In addition to the axoneme, their flagellum contains a large structure called the paraflagellar rod (PFR) whose function is unknown. We used an antisense RNA approach to produce a specific molecular ablation of the PFR structure. The mutant cells are paralysed, demonstrating that the PFR has an essential role in cell motility.

引用

收藏

页码：548 / 548

页数：1

相关论文

共 14 条

[1] A novel epitope tag system to study protein targeting and organelle biogenesis in Trypanosoma brucei [J].

Bastin, P ;

Bagherzadeh, A ;

Matthews, KR ;

Gull, K .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1996, 77 (02) :235-239

[2] The paraflagellar rod of kinetoplastida: Solved and unsolved questions [J].

Bastin, P ;

Matthews, KR ;

Gull, K .

PARASITOLOGY TODAY, 1996, 12 (08) :302-307

[3] Vectors for inducible expression of toxic gene products in bloodstream and procyclic Trypanosoma brucei [J].

Biebinger, S ;

Wirtz, LE ;

Lorenz, P ;

Clayton, C .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 85 (01) :99-112

[4] Targeting of exogenous DNA into Trypanosoma brucei requires a high degree of homology between donor and target DNA [J].

Blundell, PA ;

Rudenko, G ;

Borst, P .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1996, 76 (1-2) :215-229

[5] THE PARAFLAGELLAR ROD - A STRUCTURE IN SEARCH OF A FUNCTION [J].

CACHON, J ;

CACHON, M ;

COSSON, MP ;

COSSON, J .

BIOLOGY OF THE CELL, 1988, 63 (02) :169-181

[6]

DEFLORIN J, 1994, J BIOL CHEM, V269, P2875

[7] STABLE INTEGRATIVE TRANSFORMATION OF TRYPANOSOMA-BRUCEI THAT OCCURS EXCLUSIVELY BY HOMOLOGOUS RECOMBINATION [J].

EID, J ;

SOLLNERWEBB, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2118-2121

[8]

HYAMS JS, 1982, J CELL SCI, V55, P199

[9] Stage-specific expression of the Leishmania mexicana paraflagellar rod protein PFR-2 [J].

Moore, LL ;

Santrich, C ;

LeBowitz, JH .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1996, 80 (02) :125-135

[10] THE MAJOR COMPONENT OF THE PARAFLAGELLAR ROD OF TRYPANOSOMA-BRUCEI IS A HELICAL PROTEIN THAT IS ENCODED BY 2 IDENTICAL, TANDEMLY LINKED GENES [J].

SCHLAEPPI, K ;

DEFLORIN, J ;

SEEBECK, T .

JOURNAL OF CELL BIOLOGY, 1989, 109 (04) :1695-1709