A fluorescence-based method for detection of electron damage to DNA

被引：8
作者：
Solomun, T [1 ]
Illenberger, E [1 ]
机构：
[1] Free Univ Berlin, Inst Chem, D-14195 Berlin, Germany
来源：
CHEMICAL PHYSICS LETTERS | 2004年 / 396卷 / 4-6期
关键词：
D O I：
10.1016/j.cplett.2004.08.092
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The decomposition of DNA oligonucleotides with the sequences G(6)T(3)G(6) and G(6)(BrU)(3)G(6) (BrU = 5-bromouracil) by electrons with energy lower than the electronic excitation energy was studied by a fluorescence method. The single stranded oligonucleotides were immobilized on a gold surface in a microarray format. After the electron irradiation they were hybridized with the complementary C(6)A(3)C(6) strand marked with a dye. The fluorescence signal in positive and negative tests showed clearly the decomposition of the BrU and T oligonucleotides by low energy electrons. (C) 2004 Published by Elsevier B.V.
引用
收藏
页码：448 / 451
页数：4
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