Specific lipid requirements of membrane proteins -: a putative bottleneck in heterologous expression

被引:196
作者
Opekarová, M
Tanner, W
机构
[1] Univ Regensburg, Lehrstuhl Zellbiol & Pflanzenphysiol, D-93053 Regensburg, Germany
[2] Acad Sci Czech Republ, Inst Microbiol, CR-14220 Prague 4, Czech Republic
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2003年 / 1610卷 / 01期
关键词
lipid requirement; membrane protein; heterologous expression;
D O I
10.1016/S0005-2736(02)00708-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Membrane proteins are mostly protein-lipid complexes. For more than 30 examples of membrane proteins from prokaryotes, yeast, plant and mammals, the importance of phospolipids and sterols for optimal activity is documented. All crystallized membrane protein complexes show defined lipid-protein contacts. In addition, lipid requirements may also be transitory and necessary only for correct folding and intercellular transport. With respect to specific lipid requirements of membrane proteins, the phospholipid and glycolipid as well as the sterol content of the host cell chosen for heterologous expression should be carefully considered. The lipid composition of bacteria, archaea, yeasts, insects, Xenopus oocytes, and typical plant and mammalian cells are given in this review. A few examples of heterologous expression of membrane proteins, where problems of specific lipid requirements have been noticed or should be thought of, have been chosen. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:11 / 22
页数:12
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