Cyanine fluorochrome-labeled antibodies in vivo: Assessment of tumor imaging using Cy3, Cy5, Cy5.5, and Cy7

被引：59

作者：

Ballou, B ^{[1
]}

Fisher, GW ^{[1
]}

Deng, JS ^{[1
]}

Hakala, TR ^{[1
]}

Srivastava, M ^{[1
]}

Farkas, DL ^{[1
]}

机构：

[1] Carnegie Mellon Univ, Ctr Light Microscope Imaging & Biotechnol, Pittsburgh, PA 15213 USA

来源：

CANCER DETECTION AND PREVENTION | 1998年 / 22卷 / 03期

关键词：

animal models; fluorescence; monoclonal antibodies; photoimmunodiagnosis; renal cell carcinoma; teratocarcinoma;

D O I：

10.1046/j.1525-1500.1998.0OA25.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Monoclonal antibodies to two different targetable antigens were conjugated to each of four commercially available cyanine fluorochromes. Equal amounts of all four antibodies were coinjected into tumor-bearing animals and imaged. Small, superficial tumors were adequately labeled using all four fluorochromes. Large tumors were labeled well only by Cy7, probably due to self-masking and dilution effects. Cy7 was superior to other cyanine fluorochromes for visualizing structures located deep within the animal.

引用

页码：251 / 257

页数：7

共 19 条

[1] INTRAOPERATIVE DETECTION OF COLORECTAL-CANCER WITH RADIOIMMUNOGUIDED SURGERY AND CC49, A 2ND-GENERATION MONOCLONAL-ANTIBODY [J].

ARNOLD, MW ;

SCHNEEBAUM, S ;

BERENS, A ;

PETTY, L ;

MOJZISIK, C ;

HINKLE, G ;

MARTIN, EW .

ANNALS OF SURGERY, 1992, 216 (06) :627-632

[2]

BALLOU B, 1995, CANCER IMMUNOL IMMUN, V41, P257, DOI 10.1007/s002620050226

[3] TISSUE LOCALIZATION OF METHOTREXATE-MONOCLONAL-IGM IMMUNOCONJUGATES - ANTI-SSEA-1 AND MOPC-104E IN MOUSE TERATOCARCINOMAS AND NORMAL-TISSUES [J].

BALLOU, B ;

JAFFE, R ;

PERSIANI, S ;

SHEN, WC ;

LANGONE, JJ ;

SANDS, H ;

REILANDU, JM ;

CURLEY, J ;

HAKALA, TR .

CANCER IMMUNOLOGY IMMUNOTHERAPY, 1992, 35 (04) :251-256

[4] Three-dimensional imaging of nucleolin trafficking in normal cells, transfectants, and heterokaryons [J].

Ballou, B ;

Fisher, GW ;

Deng, JS ;

Reiland, JM ;

Hakala, TR ;

Srivastava, M ;

Farkas, DL .

ULTRASENSITIVE BIOCHEMICAL DIAGNOSTICS, PROCEEDINGS OF, 1996, 2680 :124-131

[5] TUMOR LOCATION AND DRUG TARGETING USING A MONOCLONAL-ANTIBODY (ANTI-SSEA-1) AND ANTIGEN-BINDING FRAGMENTS [J].

BALLOU, B ;

REILAND, JM ;

LEVINE, G ;

TAYLOR, RJ ;

SHEN, WC ;

RYSER, HJP ;

SOLTER, D ;

HAKALA, TR .

JOURNAL OF SURGICAL ONCOLOGY, 1986, 31 (01) :1-12

[6]

BALLOU BT, 1988, P AM ASSOC CANC RES, V29, P386

[7]

BALLOU BT, 1993, FASEB J, V7, pA1308

[8]

Ballou Byron, 1994, Proceedings of the American Association for Cancer Research Annual Meeting, V35, P504

[9]

DENG JS, 1993, J INVEST DERMATOL, V100, P527

[10] Microscopic and mesoscopic spectral bio-imaging [J].

Farkas, DL ;

Ballou, BT ;

Fisher, GW ;

Fishman, D ;

Garini, Y ;

Niu, W ;

Wachman, ES .

OPTICAL DIAGNOSTICS OF LIVING CELLS AND BIOFLUIDS, PROCEEDINGS OF, 1996, 2678 :200-209

← 1 2 →