Imaging surface structure of purple membrane partly dissolved by CHAPS with atomic force microscope

被引：2
作者：
Li, H
Chen, DL
Hu, KS
Han, BS [1 ]
机构：
[1] Chinese Acad Sci, Ctr Condensed Matter Phys, State Key Lab Magnetism, Inst Phys, Beijing 100080, Peoples R China
[2] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2003年 / 48卷 / 06期
关键词：
purple membrane; bacteriorhodopsin; detergent; CHAPS; atomic force microscope;
D O I：
10.1360/03tb9122
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Atomic force microscope was employed to study the surface structure of purple membrane (PM) using contact mode in liquid. The surface structures of native PM and PM partly dissolved by detergent CHAPS were compared. Results show that 6 mmol/L CHAPS could induce some cracks and holes in PM. While Bateriorhodopsin (BR) was still presented as trimers in the two-dimensional hexagonal structure. We assume that it was caused by the interaction between CHAPS micellar and PM. Both absorption spectra and flash photolysis kinetic spectra indicated that BR's biochemical functions have been changed.
引用
收藏
页码：573 / 576
页数：4
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