Molecular detection of Helicobacter pylori and clarithromycin resistance in gastric biopsies: a prospective evaluation of RIDA®GENE Helicobacter pylori assay

被引：13

作者：

Van den Poel, Bea ^{[1
]}

Gils, Sarah ^{[1
]}

Micalessi, Isabel ^{[1
]}

Carton, Saskia ^{[2
]}

Christiaens, Paul ^{[2
]}

Cuyle, Pieter-Jan ^{[2
]}

Moons, Veerle ^{[2
]}

Van Olmen, Gust ^{[2
]}

Smismans, Annick ^{[1
]}

Bourgain, Claire ^{[3
]}

Bossuyt, Peter ^{[2
]}

Frans, Johan ^{[1
]}

机构：

[1] Imelda Gen Hosp, Dept Lab Med, Bonheiden, Belgium

[2] Imelda Gen Hosp, Dept Gastroenterol, Bonheiden, Belgium

[3] Imelda Gen Hosp, Dept Pathol, Bonheiden, Belgium

来源：

ACTA CLINICA BELGICA | 2021年 / 76卷 / 03期

关键词：

Helicobacter pylori; qPCR; clarithromycin resistance; gastric biopsy; RIDA (R) GENE Helicobacter pylori; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; ANTIBIOTIC-RESISTANCE; INFECTION; ERADICATION; MANAGEMENT; CONSENSUS; STOOL;

D O I：

10.1080/17843286.2019.1685741

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Empirical treatment of Helicobacter pylori (HP) depends on the local prevalence of clarithromycin resistance but data are lacking and culturing of HP is time-consuming. We evaluated RIDA (R) GENE Helicobacter pylori assay (r-biopharm), a quantitative PCR assay for detecting HP and clarithromycin resistance mutations in gastric biopsies. Material/methods: Gastric biopsies were obtained from each of 436 consecutive patients referred for gastroscopic investigation and results of qPCR were compared to culture and immunohistochemical staining (IHCS). Results: Of 436 samples, 47 were positive for HP by PCR (11%), 42 by culture (9.7%) and 44 by IHCS (10%). Compared to culture, sensitivity and specificity of the qPCR were 100% and 99%, respectively, and 96% and 99% compared to IHCS. The sensitivity and specificity for clarithromycin resistance detection were 92% and 97%, respectively. Conclusions: RIDA (R) GENE Helicobacter pylori assay reliably and rapidly detects HP and its resistance to clarithromycin in human gastric biopsies.

引用

页码：177 / 183

页数：7

共 39 条

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