Selenium incorporation using recombinant techniques

被引：59

作者：

Walden, Helen ^{[1
]}

机构：

[1] Canc Res UK London Res Inst, Prot Struct & Funct Lab, London WC2A 3PX, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2010年 / 66卷

关键词：

SELENOMETHIONINE-LABELED PROTEINS; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; CRYSTALLIZATION; EXPRESSION; SYNTHASE; MAD; AUTOINDUCTION; COMPLEXES; EFFICIENT;

D O I：

10.1107/S0907444909038207

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Using selenomethionine to phase macromolecular structures is common practice in structure determination, along with the use of selenocysteine. Selenium is consequently the most commonly used heavy atom for MAD. In addition to the well established recombinant techniques for the incorporation of selenium in prokaryal expression systems, there have been recent advances in selenium labelling in eukaryal expression, which will be discussed. Tips and things to consider for the purification and crystallization of seleno-labelled proteins are also included.

引用

页码：352 / 357

页数：6

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