DNAzyme self-assembled gold nanoparticles for determination of metal ions using fluorescence anisotropy assay

被引：110

作者：

Yin, Bin-Cheng ^{[1
]}

Zuo, Peng ^{[1
]}

Huo, Hao ^{[1
]}

Zhong, Xinhua ^{[1
]}

Ye, Bang-Ce ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 401卷 / 01期

基金：

美国国家科学基金会;

关键词：

DNAzyme; Gold nanoparticles; Copper; Lead; Fluorescence anisotropy; CATALYTIC BEACON SENSOR; AU-NANOPARTICLES; AMPLIFIED DETECTION; OPTICAL-DETECTION; COPPER IONS; DNA; LEAD; SENSITIVITY; SELECTIVITY; MONOLAYERS;

D O I：

10.1016/j.ab.2010.02.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gold nanoparticles can be exploited to facilitate a highly sensitive and selective metal ion detection based on fluorescence anisotropy assay with metal ion-dependent DNA-cleaving DNAzyme. This assay allows rapid and accurate determination of metal ions in aqueous medium at room temperature. The method has been demonstrated for determination of Cu2+ and Pb2+ ions. The detection sensitivity can be significantly improved to 1 nM by using a "nanoparticle enhancement" approach. Moreover, the assay was also tested in 384-well plates for high-throughput routine determination of toxic metal ions in environmental samples. The method showed distinct advantages over conventional methods in terms of its potential sensitivity, specificity, and ability for rapid response. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：47 / 52

页数：6

共 33 条

[1] Direct determination of cadmium and copper in seawater using a transversely heated graphite furnace atomic absorption spectrometer with Zeeman-effect background corrector [J].