Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy

被引：76

作者：

Chua, Eugene Y. D. ^{[1
,2
,3
]}

Mendez, Joshua H. ^{[1
,2
,3
]}

Rapp, Micah ^{[1
,2
]}

Ilca, Serban L. ^{[1
,2
]}

Tan, Yong Zi ^{[7
,8
]}

Maruthi, Kashyap ^{[1
,2
,4
]}

Kuang, Huihui ^{[1
,2
,4
]}

Zimanyi, Christina M. ^{[1
,2
,3
]}

Cheng, Anchi ^{[1
,2
,4
]}

Eng, Edward T. ^{[1
,2
,3
]}

Noble, Alex J. ^{[1
,2
,4
,5
,6
]}

Potter, Clinton S. ^{[1
,2
,3
,4
,5
,6
]}

Carragher, Bridget ^{[1
,2
,3
,4
,5
,6
]}

机构：

[1] New York Struct Biol Ctr, New York, NY 10027 USA

[2] Simons Electron Microscopy Ctr, New York, NY 10027 USA

[3] Natl Ctr CryoEM Access & Training, New York, NY 10027 USA

[4] Natl Resource Automated Mol Microscopy, New York, NY USA

[5] Natl Ctr In Situ Tomog Ultramicroscopy, New York, NY USA

[6] Simons Machine Learning Ctr, New York, NY USA

[7] Natl Univ Singapore, Dept Biol Sci, Singapore, Singapore

[8] ASTAR, Dis Intervent Technol Lab, Singapore, Singapore

来源：

ANNUAL REVIEW OF BIOCHEMISTRY | 2022年 / 91卷

基金：

美国国家卫生研究院;

关键词：

CRYO-EM STRUCTURES; BEAM-INDUCED MOTION; ELECTRON-DIFFRACTION DATA; STRUCTURE PREDICTION; SAMPLE PREPARATION; GRID PREPARATION; DATA-ACQUISITION; IN-SITU; REFINEMENT; RESOLUTION;

D O I：

10.1146/annurev-biochem-032620-110705

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cryo-electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stage of the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.

引用

页码：1 / 32

页数：32

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