DMSP Production by Coral-Associated Bacteria

被引:22
作者
Kuek, Felicity W. I. [1 ,2 ,3 ,4 ,5 ]
Motti, Cherie A. [1 ,5 ]
Zhang, Jia [3 ,4 ]
Cooke, Ira R. [3 ,4 ]
Todd, Jonathan D. [6 ]
Miller, David J. [2 ,3 ,4 ]
Bourne, David G. [1 ,5 ,7 ]
Raina, Jean-Baptiste [8 ]
机构
[1] James Cook Univ, Div Res & Innovat, AIMS JCU, Townsville, Qld, Australia
[2] James Cook Univ, Australian Res Council ARC Ctr Excellence Coral Re, Townsville, Qld, Australia
[3] James Cook Univ, Ctr Trop Bioinformat & Mol Biol, Townsville, Qld, Australia
[4] James Cook Univ, Coll Publ Hlth Med & Vet Sci, Townsville, Qld, Australia
[5] Australian Inst Marine Sci, Trop Marine Water Qual & Impacts, Townsville, Qld, Australia
[6] Univ East Anglia, Sch Biol Sci, Norwich Res Pk, Norwich, England
[7] James Cook Univ, Coll Sci & Engn, Townsville, Qld, Australia
[8] Univ Technol Sydney, C3, Ultimo, NSW, Australia
关键词
DMSP; sulfur cycle; coral-associated bacteria; holobiont; acrylate; REEF-BUILDING CORAL; DIMETHYLSULFONIOPROPIONATE DMSP; OXIDATIVE STRESS; SP-NOV; SEQUENCE; DIMETHYLSULFIDE; SALINITY; SEARCH; BIOSYNTHESIS; DIVERSITY;
D O I
10.3389/fmars.2022.869574
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Dimethylsulfoniopropionate (DMSP) is an important molecule in the marine sulfur cycle, produced in large amounts by corals and their dinoflagellate endosymbionts, Symbiodiniaceae. Although corals are known to harbour bacteria that can catabolise DMSP, the recent discovery of bacteria capable of producing DMSP in coastal and deep-sea environments raises the possibility of a bacterial contribution to the DMSP output of corals. Here, 157 bacteria associated with four common coral species were isolated and screened for their ability to produce DMSP by targeting dsyB, a key gene involved in DMSP biosynthesis. Approximately 9% (14 out of 157) of the bacterial isolates harboured dsyB, all being members of the Alphaproteobacteria. The ability of these isolates to produce DMSP was confirmed by liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) measurements. A dsyB-harbouring strain, Shimia aestuarii AMM-P-2, was selected for genome sequencing. This strain harbours the complete genetic machinery to (i) assimilate sulfate and synthesise the DMSP precursors, cysteine and methionine; (ii) demethylate DMSP and generate methanethiol; (iii) cleave DMSP, generating dimethyl sulfide (DMS) and acrylate; and (iv) utilise or detoxify acrylate. The impacts of varied environmental factors (temperature, salinity, light and UV radiation) on S. aestuarii AMM-P-2 DMSP biosynthesis were characterised. DMSP levels in S. aestuarii AMM-P-2 increased almost two-fold under both hypersaline conditions (40 PSU) and high UV exposure. DMSP catabolism through the cleavage pathway also increased under these conditions, producing the antioxidants DMS and acrylate, a potential response to the oxidative stress generated. Overall, our results reveal that coral-associated bacteria can synthesize DMSP and may therefore contribute to DMSP production by the coral holobiont.
引用
收藏
页数:12
相关论文
共 82 条
  • [1] Transcriptomic analysis of the response of Acropora millepora to hypo-osmotic stress provides insights into DMSP biosynthesis by corals
    Aguilar, Catalina
    Raina, Jean-Baptiste
    Motti, Cherie A.
    Foret, Sylvain
    Hayward, David C.
    Lapeyre, Bruno
    Bourne, David G.
    Miller, David J.
    [J]. BMC GENOMICS, 2017, 18
  • [2] Improvement of the ERETIC method by digital synthesis of the signal and addition of a broadband antenna inside the NMR probe
    Akoka, S
    Trierweiler, M
    [J]. INSTRUMENTATION SCIENCE & TECHNOLOGY, 2002, 30 (01) : 21 - 29
  • [3] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [4] ANDREAE MO, 1983, ECOL BULL, P167
  • [5] Fe-s cluster assembly pathways in bacteria
    Ayala-Castro, Carla
    Saini, Avneesh
    Outten, F. Wayne
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2008, 72 (01) : 110 - +
  • [6] SEASONAL RELATIONSHIP BETWEEN CLOUD CONDENSATION NUCLEI AND AEROSOL METHANESULFONATE IN MARINE AIR
    AYERS, GP
    GRAS, JL
    [J]. NATURE, 1991, 353 (6347) : 834 - 835
  • [7] SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
    Bankevich, Anton
    Nurk, Sergey
    Antipov, Dmitry
    Gurevich, Alexey A.
    Dvorkin, Mikhail
    Kulikov, Alexander S.
    Lesin, Valery M.
    Nikolenko, Sergey I.
    Son Pham
    Prjibelski, Andrey D.
    Pyshkin, Alexey V.
    Sirotkin, Alexander V.
    Vyahhi, Nikolay
    Tesler, Glenn
    Alekseyev, Max A.
    Pevzner, Pavel A.
    [J]. JOURNAL OF COMPUTATIONAL BIOLOGY, 2012, 19 (05) : 455 - 477
  • [8] EVIDENCE FOR THE CLIMATIC ROLE OF MARINE BIOGENIC SULFUR
    BATES, TS
    CHARLSON, RJ
    GAMMON, RH
    [J]. NATURE, 1987, 329 (6137) : 319 - 321
  • [9] Bacterial Communities of Diatoms Display Strong Conservation Across Strains and Time
    Behringer, Gregory
    Ochsenkuhn, Michael A.
    Fei, Cong
    Fanning, Jhamal
    Koester, Julie A.
    Amin, Shady A.
    [J]. FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [10] Trimmomatic: a flexible trimmer for Illumina sequence data
    Bolger, Anthony M.
    Lohse, Marc
    Usadel, Bjoern
    [J]. BIOINFORMATICS, 2014, 30 (15) : 2114 - 2120