Chemical composition and effects of Ocimum gratissimum essential oil (OGEO) on the expression of mRNA for antioxidant enzymes during in vitro culture of bovine ovarian secondary follicles

被引：0

作者：

Vasconcelos, E. M. ^{[1
]}

Costa, F. C. ^{[1
]}

Azevedo, V. A. N. ^{[1
]}

Paulino, L. R. F. M. ^{[1
]}

Soares, M. D. ^{[1
]}

Souza, A. L. P. ^{[1
]}

Carneiro, V. A. ^{[2
]}

Brito, G. S. ^{[3
]}

Catunda Junior, F. E. A. ^{[3
]}

Silva, J. R. V. ^{[1
]}

机构：

[1] Univ Fed Ceara, Lab & Biotechnol & Physiol Reprod LABIREP, Sobral, Brazil

[2] Univ Fed Ceara, Lab Biofilms & Antimicrobial Agents, Sobral, Brazil

[3] State Univ Tocantina Reg Maranhao, Ctr Exact Nat & Technol Sci, Imperatriz, Brazil

来源：

JOURNAL OF ESSENTIAL OIL RESEARCH | 2022年 / 34卷 / 05期

关键词：

antioxidant; essential oil; preantral follicles; bovine; in vitro culture; MATURATION; LOCALIZATION; QUALITY; OOCYTES; STRESS; LEAVES; MEDIA; TIME;

D O I：

10.1080/10412905.2022.2067256

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study analyzed the chemical composition of OGEO and its effects on growth, viability, antrum formation and mRNA expression for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and peroxiredoxin 6 (Prdx6) in bovine secondary follicles cultured in vitro. The follicles were cultured in TCM-199+ alone or supplemented with 0.125, 0.25 and 0.50% OGEO for 18 days. Follicle diameters, viability, antrum formation and the levels of mRNA for SOD, CAT, GPX1 and PRDX6 in cultured follicles were evaluated. The results showed that the main components of OGEO were Eugenol and 1.8-Cineol. The follicles cultured with OGEO maintained morphology, viability and growth similar to the control group. Interestingly, follicles cultured with 0.5% or 0.25% OGEO had, respectively, higher levels of mRNAs for PRDX6 and CAT than those cultured in control medium. In conclusion, OGEO increases mRNA expression for PRDX6 and CAT in bovine secondary follicles cultured in vitro.

引用

页码：446 / 454

页数：9

共 17 条

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