Studies on the separation and extraction of α-amylase and proteinase from fermented liquor of α-amylase by aqueous two-phase partitioning

被引：0

作者：

Jiao, QC ^{[1
]}

Liu, Q

Chen, YZ

机构：

[1] Nanjing Univ, Dept Biol Sci & Technol, Nanjing 210093, Peoples R China

[2] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 1998年 / 19卷 / 03期

关键词：

alpha-amylase; proteinase; aqueous two-phase system; polyethylene glycol; extraction; back-extraction;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Aqueous two-phase system composed of PEG/(NH4)(2)SO4 was once to isolate alpha-amylase and proteinase from fermented liquor of alpha-amylase in this paper. The optimum extraction conditions were: 15% PEG1000, 20% (NH4)(2)SO4, pH=8, the partition coefficient of alpha-amylase was as high as 19.6 with a yield of 90%. The separation coefficients between alpha-amylase and proteinase were 15.1 and 1.5 folds increase of specific activity compared with those the original fermented liquor. The yield of proteinase in water phase was more than 60%. The preliminary study on back-extraction condition was done in this paper.

引用

页码：391 / 394

页数：4