MicroRNA-resistant alleles of HOMEOBOX DOMAIN-2 modify inflorescence branching and increase grain protein content of wheat

被引：24

作者：

Dixon, Laura E. ^{[1
,2
]}

Pasquariello, Marianna ^{[1
]}

Badgami, Roshani ^{[1
]}

Levin, Kara A. ^{[3
]}

Poschet, Gernot ^{[4
]}

Ng, Pei Qin ^{[5
]}

Orford, Simon ^{[6
]}

Chayut, Noam ^{[6
]}

Adamski, Nikolai M. ^{[1
]}

Brinton, Jemima ^{[1
]}

Simmonds, James ^{[1
]}

Steuernagel, Burkhard ^{[1
]}

Searle, Iain R. ^{[5
]}

Uauy, Cristobal ^{[1
]}

Boden, Scott A. ^{[1
,3
]}

机构：

[1] John Innes Ctr, Dept Crop Genet, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

[2] Univ Leeds, Fac Biol Sci, Leeds LS2 9JT, W Yorkshire, England

[3] Univ Adelaide, Sch Agr Food & Wine, Waite Res Inst, Glen Osmond, SA 5064, Australia

[4] Heidelberg Univ, Ctr Organismal Studies COS, D-69120 Heidelberg, Germany

[5] Univ Adelaide, Sch Biol Sci, Adelaide, SA 5005, Australia

[6] John Innes Ctr, Germplasm Resources Unit, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

来源：

SCIENCE ADVANCES | 2022年 / 8卷 / 19期

基金：

澳大利亚研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

HOMEODOMAIN-LEUCINE-ZIPPER; GENE FAMILY-MEMBERS; ORGAN DEVELOPMENT; HIDDEN VARIATION; FRIZZY-PANICLE; ARABIDOPSIS; MAIZE; ARCHITECTURE; EXPRESSION; TARGETS;

D O I：

10.1126/sciadv.abn5907

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plant and inflorescence architecture determine the yield potential of crops. Breeders have harnessed natural diversity for inflorescence architecture to improve yields, and induced genetic variation could provide further gains. Wheat is a vital source of protein and calories; however, little is known about the genes that regulate the development of its inflorescence. Here, we report the identification of semidominant alleles for a class III homeodomain-leucine zipper transcription factor, HOMEOBOX DOMAIN-2 (HB-2), on wheat A and D subgenomes, which generate more flower-bearing spikelets and enhance grain protein content. These alleles increase HB-2 expression by disrupting a microRNA 165/166 complementary site with conserved roles in plants; higher HB-2 expression is associated with modified leaf and vascular development and increased amino acid supply to the inflorescence during grain development. These findings enhance our understanding of genes that control wheat inflorescence development and introduce an approach to improve the nutritional quality of grain.

引用

页数：16

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