Total Fractionation and Analysis of Polysaccharides from Leaves of Panax ginseng C. A. Meyer

被引：0
作者：
Ni Xiu-zhen [1 ,2 ]
Wang Bing-qing [1 ]
Zhi Yuan [1 ]
Wei Ning-ning [1 ]
Zhang Xu [1 ]
Li Shan-shan [1 ]
Tai Gui-hua [1 ]
Zhou Yi-fa [1 ]
Zhao Ji-ming [2 ]
机构：
[1] NE Normal Univ, Sch Life Sci, Changchun 130024, Peoples R China
[2] Changchun Normal Univ, Sch Life Sci, Changchun 130032, Peoples R China
来源：
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES | 2010年 / 26卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Ginseng leave; Polysaccharide; Fractionation; RG-I domain;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The water-soluble polysaccharides isolated from the leaves of Panax ginseng C. A. Meyer were totally fractionated into one neutral fraction(GLPN-1); six acidic fractions(GLPN-2, GLPA-1a, GLPA-1b, GLPA-1c, GLPA-2 and GLPA-3) by a combination of ethanol precipitation, ion-exchange and gel permeation chromatography. All the fractions were analyzed by determining monosaccharide composition, molecular weight distribution. The results show that GLPN-1 was mainly composed of Gal(38.1%) and Ara(33.6%) and presumed to be an arabinogalactan. GLPA-1a, GLPA-1b and GLPA-2 mainly contained Gal, Ara, Rha and GalA. The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%. The molar ratios of Rha/GalA in GLPA-1a, GLPA-1b and GLPA-2 were 1:1, 1:2 and 1:2.7, respectively, indicating that they belong to type-I rhamnogalacturonan (RG-I)-rich pectin. GLPA-1c was mainly composed of GalA(78%), belonging to homogalacturonan(HG)-rich pectin. GLPA-3 might be a glycoprotein.
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页码：230 / 234
页数：5
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