An integrated genetic and man-mouse comparative map of the DXHXS674-Pdha1 region of the mouse X chromosome

The genes for ocular albinisim type I (OA1) and the Xenopus Laevis-like apical protein (APXL) map between amelogenin (AMELX) and the pseudoautosomal boundary in the distal region of the human X chromosome short arm. The mouse homologues, Oa1 and Apxl, have recently been shown to lie proximal to their expected locations an the mouse X chromosome, but their positions with respect to critical gene loci in the vicinity have not been defined. By analyzing recombination events Tram (Mus musculus x Mus spretus) x M. musculus backcrosses, we have constructed a detailed mouse genetic map that encompasses Oa1, five other genes, and 13 microsatellite loci. The order of genes and evolutionary breakpoints (EB) is defined as centromere-(EB)-(DXHXS674, DXHXS679)-Smcx-(EB)-Oa1-(EB)-Phex (3' --> 5')-Pdha1-telomere. Thus Oa1 lies in a region between two previously characterized conserved segments. (C) 1998 Academic Press.