Endothelin-B receptors on suprachoroidal melanocytes mediate an endothelin-1-induced increase in the intracellular calcium concentration of rabbit ocular suprachoroidal tissue

被引：6
作者：
Hirose, Akira
Azuma, Hiroshi
Tokoro, Takashi
Hirai, Keiji
机构：
[1] Univ Tokyo, Dept Ophthalmol, Grad Sch Med, Bunkyo Ku, Tokyo 1138655, Japan
[2] Tokyo Med & Dent Univ, Dept Ophthalmol & Visual Sci, Grad Sch Med, Tokyo, Japan
[3] Tokyo Med & Dent Univ, Dept Biosyst Regulat, Inst Biomat & Bioengn, Grad Sch, Tokyo, Japan
[4] Tokyo Med & Dent Univ, Dept Autonom Physiol, Med Res Inst, Tokyo, Japan
来源：
CURRENT EYE RESEARCH | 2007年 / 32卷 / 06期
关键词：
endothelin-1; endothelin-B receptor; fura-2; microfluorophotometry; immuno-electron microscopy; suprachoroidal melanocytes;
D O I：
10.1080/02713680701391529
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
The physiological properties of endothelin-1 in ocular choroidal melanocytes are not well-known, although endothelin-1 increases the intracellular calcium concentration through endothelin-B receptors in skin melanocytes. We studied the effect of endothelin-1 on choroidal melanocytes in rabbit ocular suprachoroidal tissue. Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue. Endothelin-B receptor selective antagonist BQ788 blunted the response. Immuno-electron microscopy showed that endothelin-B receptor-like immunoreactivity was located only on melanocytes. These results indicate that endothelin-1 increases intracellular calcium concentration in rabbit suprachoroidal tissue via endothelin-B receptors on melanocytes.
引用
收藏
页码：585 / 591
页数：7
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