Cloning and functional expression of intracellular loop variants of the honey bee (Apis mellifera) RDL GABA receptor

被引:20
作者
Taylor-Wells, Jennina [1 ,2 ]
Hawkins, Joseph [1 ]
Colombo, Claudia [1 ]
Bermudez, Isabel [1 ]
Jones, Andrew K. [1 ]
机构
[1] Oxford Brookes Univ, Fac Hlth & Life Sci, Dept Biol & Med Sci, Oxford OX3 0BP, England
[2] Univ Florida, Dept Entomol & Nematol, Emerging Pathogens Inst, Gainesville, FL 32610 USA
关键词
Apis mellifera; Fipronil; GABA receptor; Imidacloprid; Splice variant; Xenopus oocytes; NICOTINIC ACETYLCHOLINE-RECEPTORS; GAMMA-2; SUBUNIT; SPLICE-VARIANT; INSECTICIDES; DROSOPHILA; RESISTANCE; FIPRONIL; MUTATION; PHOSPHORYLATION; IDENTIFICATION;
D O I
10.1016/j.neuro.2016.06.007
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The insect GABA receptor, RDL (resistance to dieldrin), plays central roles in neuronal signalling and is the target of several classes of insecticides. To study the GABA receptor from an important pollinator species, we cloned Rdl cDNA from the honey bee, Apis mellifera. Three Rdl variants were identified, arising from differential use of splice acceptor sites in the large intracellular loop between transmembrane regions 3 and 4. These variants were renamed from previously, as Amel_RDLvar1, Amel_RDLvar2 and Amel_RDLvar3. When expressed in Xenopus laevis oocytes, the three variants showed no difference in sensitivity to the agonist, GABA, with EC(50)s of 29 mu M, 20 mu M and 29 mu M respectively. Also, the potencies of the antagonists, fipronil and imidacloprid, were similar on all three variants. Fipronil IC50 values were 0.18 mu M, 0.31 mu M and 0.20 mu M whereas 100 mu M imidacloprid reduced the GABA response by 17%, 24% and 31%. The possibility that differential splicing of the RDL intracellular loop may represent a species specific mechanism leading to insensitivity to insecticides is discussed. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:207 / 213
页数:7
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